Assay Kits  >  Viral Protease Assay Kits - HIV, HCV, WNV  >>  SensoLyte^® 520 HIV Protease Assay Kit *Fluorimetric*

The SensoLyte® 520 HIV Assay Kit uses a new FRET peptide substrate that incorporates HiLyte Fluor™ 488 (fluorophore) and QXL™ 520 (quencher) for continuous measurement of enzyme activities. In the intact FRET peptide, the fluorescence of HiLyte Fluor™ 488 is quenched by QXL™ 520. Upon cleavage of the FRET peptide by HIV protease, the fluorescence of HiLyte Fluor™ 488 is recovered and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, this HiLyte Fluor™ 488/QXL™ 520-based FRET peptide shows less interference from autofluorescence of test compounds and cellular components, thus providing better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format. The kit contains: • HiLyte Fluor™ 488/QXL™ 520-based FRET peptide substrate (Ex/Em=490/520 nm upon cleavage) • Assay buffer • HIV protease inhibitor • Fluorescence reference standard for calibration • A detailed protocol

Kit Size: 100 assays