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Assay Kits  >  Viral/Bacterial Protease Assay Kits - HIV, HCV, TEV, WNV, Sortase  >>  SensoLyte® 490 HCV Protease Assay Kit *Fluorimetric*

Product Name SensoLyte® 490 HCV Protease Assay Kit *Fluorimetric*
Kit Size: 500 assays
Size 1 kit
Catalog # AS-71126
US$ $631
Description

HCV protease is identified as an important drug-screening target. The SensoLyte® 490 HCV Protease Assay Kit uses an optimized FRET peptide substrate for the continuous measurement of HCV NS3/4a activity. This FRET substrate is cleaved specifically by HCV NS3/4a protease thereby liberating the C-terminal peptide-fluorophore fragment from the proximity quenching effect of the dark quencher, resulting in a more than 10-fold increase in fluorescence. This assay can detect HCV NS3/4a protease activity at 1 pmole. The kit contains: • EDANS/DABCYL-based FRET peptide substrate (Ex/Em=340/490 nm upon cleavage) • Assay buffer • Fluorescence reference standard for calibration • A detailed protocol

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Poster
Brochure
Product Citations Park, HR. et al. (2011). 2-arylmethylaminomethyl-5,6-dihydroxychromone derivatives with selective anti-HCV activity. Bioorg Med Chem Lett 10.1016/j.bmcl.2011.04.055.
Peng, LF. et al. (2011). Ceestatin, a novel small molecule inhibitor of hepatitis C virus replication, inhibits 3-hydroxy-3-methylglutaryl-coenzyme A synthase. J Infect Dis 204, 609.
Yon, C. et al. (2011). Mutations in HCV non-structural genes do not contribute to resistance to nitazoxanide in replicon-containing cells. Antiviral Res., 91, 233.
Frick, D. et al. (2010). A Method to Simultaneously Monitor Hepatitis C Virus NS3 Helicase and Protease Activities. Meth Mol Biol 587, 223.
     
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