390 MMP FRET Substrate I Mca - PLGL - Dpa - AR - NH2
% Peak Area By HPLC ≥ 95%
A fluorogenic substrate that is best cleaved by MMP-7, 8, 9 and 13. The highly fluorescent Mca moiety is efficiently quenched by energy transfer to the Dnp group. The punctuated metallo proteinase (PUMP, EC 22.214.171.124) cleaves the substrate at the Gly-Leu bond with a 190-fold increase in fluorescence (Abs/Em = 325/393 nm). In human matrix metalloproteinases assays, this substrate is about 50 to 100 times more sensitive than the generic MMP substrate, Dnp-PLGLWAr-NH2 (Cat# 27074).