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Assay Kits  >  Caspase Assay Kits  >>  SensoLyteŽ Homogeneous AFC Caspase-3/7 Assay Kit

Product Name SensoLyteŽ Homogeneous AFC Caspase - 3/7 Assay Kit
500 assays (96 - well plate)
Size 1 kit
Catalog # AS-71114
US$ $272
Description

Central to the execution phase of apoptosis are the two closely related caspase-3 and caspase-7. They share common substrate specificity and structure, but differ completely in the sequence of their respective N-terminal regions. Both caspase-3 and caspase-7 have substrate selectivity for the amino acid sequence Asp-Glu-Val-Asp (DEVD).

The SensoLyteŽ Homogeneous AFC Capase-3/7 Assay Kit uses Ac-DEVD-AFC as the fluorogenic indicator for assaying caspase-3/7 activities. Upon caspase-3/7 cleavage, Ac-DEVD-AFC generates the AFC fluorophore which has bright blue fluorescence and can be detected at Ex/Em=380 nm/500 nm. A bi-function assay buffer in this kit is designed to lyze the cells and measure the enzyme activity at the same time. Thus, this kit can measure caspase-3/7 activity in cell culture directly in a 96-well or 384-well plate without a time-consuming cell extraction step. In case the cells are cultured in larger plates or flasks, a lysis buffer and protocol for cell lysate preparation are also conveniently included in the kit. The kit is suitable for high throughput screening of apoptosis inducers and inhibitors.

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Poster
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Freise, C. et al. (2012). The inhibitor of Ca2+-dependent K+ channels TRAM-34 blocks growth of hepatocellular carcinoma cells via downregulation of estrogen receptor alpha mRNA and nuclear factor-kappaB. Invest New Drugs. doi:10.1007/s10637-012-9879-6
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Cheung, M. et al. (2010). An evolved ribosome-inactivating protein targets and kills human melanoma cells in vitro and in vivo. Mol Cancer 10.1186/1476-4598-9-28.
Freise, C. et al. (2009). An active extract of Lindera obtusiloba inhibits adipogenesis via sustained Wnt signaling and exerts anti-inflammatory effects in the 3T3-L1 preadipocytes. J Nutr Biochem 10.1016/j.jnutbio.2009.09.013.
Rueh, M. et al. (2009). Extracts of Lindera obtusiloba induce antifibrotic effects in hepatic stellate cells via suppression of a TGF-β-mediated profibrotic gene expression pattern. J Nutr Biochem 20, 597.
Ou, D. et al. (2005). Synergistic inhibition of tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis in human pancreatic β cells by Bcl-2 and X-linked inhibitor of apoptosis. Hum Immunol 66, 274.
     
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