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Fluorescent Dyes  >  Labeling and Detection of Peptides and Proteins  >>  NBD-Cl

Product Name NBD - Cl
[4 - Chloro - 7 - nitrobenzofurazan] *UltraPure Grade*
Size 100 mg
Catalog # AS-81203
US$ $66
Description

NBD-Cl is non-fluorescent, and generates fluorescent products upon reacting with aliphatic amines or thiol compounds. It reacts with amino groups such as aliphatic amines, amino acids, peptides, and proteins to form highly fluorescent compounds. The fluorescence spectra of NBD-amine adducts are highly environment-sensitive, the fluorescence intensity decreases significantly in aqueous solutions. The NBD-amine adducts have lex = 464 nm and lem = 512 nm in aqueous solutions. NBD-Cl also reacts with thiol group to form fluorescent adducts. NBD-Cl is widely used to label peptides, proteins, drugs and other biomolecules. It is also a popular derivatizing reagent for HPLC analysis.

Minimum Purity: 98%

Detailed Information Material Safety Data Sheets (MSDS)
Storage -20C desiccated and protected from light
References Babia T, et al. (2001). Endocytosis of NBD-sphingolipids in neurons: exclusion from degradative compartments and transport to the Golgi complex. Traffic 2, 395-405; Schramm U, et al. (1993). Fluorescent derivatives of bile salts. III. Uptake of 7 beta-NBD-NCT into isolated hepatocytes by the transport systems for cholyltaurine. J Lipid Res 34, 741-57.
Molecular Weight 199.6
Spectral Properties Abs/Em = 337/none nm
Solvent System DMF or DMSO
     
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