Species Reactivity: Human, mouse, rat; others not tested.
Product Description: Rabbit polyclonal anti-p53 (CT) antibody is supplied as an epitope affinity purified rabbit IgG, 50 µg in 250 µl 1x PBS (pH 7.4) containing 0.05% sodium azide.
Immunogen: Rabbit polyclonal anti-p53 (CT) antibody was raised against a synthetic peptide (KKGQSTS), corresponding to human p53 and surrounding the C-terminal (CT) amino acids.
Application Notes: The following concentration ranges are recommended starting points. Optimal working concentrations for specific applications should be determined by the investigator.
ELISA for immunizing peptide: 1: 5,000-20,000 WB: 1: 500-2,000
Figure 1. Western blot of human p53 in MCF-7 cell lysate probed with rabbit polyclonal Anti-p53 (CT) antibody (Cat# 54421) at 1 µg/ml. An immunoreactive band was detected at ~53 kDa. Goat anti-rabbit IgG (H+L), highly cross-adsorbed, HiLyte Fluor™ 750-labeled secondary antibody (Cat# 61056-05-H750 or 61056-1-H750) was used and the signals detected using an infrared-imaging system (Odyssey, Li-Cor).
Figure 2. Western blot of human p53 in hydroxyrea treated Cos-7 cell lysate labeled with rabbit polyclonal Anti-p53 (CT) antibody (Cat# 54421) at 1 µg/ml. An immunoreactive band was detected at ~53 kDa.