This peptide corresponds to the 14-21 amino acid residues of Histone 4 in which lysine at 16th position is acetylated and the peptide is labeled with a fluorophore, (7-Methoxycoumarin-4-yl)acetyl (Mca). Labeling peptides with Mca fluorophore confers applications in protein-protein interaction and localization studies. The H4K16Ac peptide has been studied in relation to the male-specific lethal (MSL) complex wherein when the MSL complex associates with activated genes, an mof histone acetyltransferase acetylates H4 at lysine 16 throughout transcriptional domains, favoring the 3' end of transcription units over promoters. This, and further studies based on the distribution of H4K16Ac in X-linked transcriptional domains has led to the conclusion that chromatin modifications responsible for dosage compensation is due to the rate of enhancement in the rate of transcription elongation, which is nothing but an increase in the extension rate of RNA polymerase.
Yokoyama R. et al. Mol Cell Biol, 27(22):7865-7870 (2007).
Sequence (One-Letter Code)
Sequence (Three-Letter Code)
Mca - Gly - Ala - Lys(Ac) - Arg - His - Arg - Lys - Val - NH2