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Recombinant Proteins  >  ADAMTS/Aggrecanases  >>  SensoLyte® 520 Aggrecanase-1 Assay Kit *Fluorimetric*

Product Name SensoLyte® 520 Aggrecanase - 1 Assay Kit *Fluorimetric*
Size 1 kit
Catalog # 72114
US$ $435
Description

Aggrecanases belong to ADAMTS (A disintegrin and metalloprotease with thrombospondin motif) family of proteases. Aggrecanases cleave aggrecan, the major structural component of cartilage. Aggrecanase-1 (ADAMTS-4) is a major aggrecanase in human osteoarthritic cartilage.

The SensoLyte® 520 Aggrecanase-1 Assay Kit uses 5-FAM as the fluorophore and TAMRA as the quencher in the FRET peptide substrate. This substrate is used for continuous measurement of the enzyme activity. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by TAMRA. Upon the cleavage of the FRET peptide by ADAMTS-4, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. Increase in FAM fluorescence is correlated to aggrecanase-1 activity. 5-FAM/TAMRA based FRET substrate is less interfered by autofluorescence of test compounds due to its longer emission wavelength. The kit can be used to detect the activity of aggrecanase-1 or screen for aggrecanase inhibitors. The assays are performed in a convenient 96-well format.

Kit size: 100 assays

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Poster
Storage -20°C
Product Citations Wainwright, SD. et al. (2013). ADAMTS4_v1 is a splice variant of ADAMTS4 that is expressed as a protein in human synovium and cleaves aggrecan at the interglobular domain. Arthritis Rheumatism 65, 2866. doi: 10.1002/art.38102.
Tauchi, R. et al. (2012). The endogenous proteoglycan-degrading enzyme ADAMTS-4 promotes functional recovery after spinal cord injury. J Neuroinflammation 9, 53.
Moncada-Pazos, A. et al. (2011). The nutraceutical flavonoid luteolin inhibits ADAMTS-4 and ADAMTS-5 aggrecanase activities. J Mol Med DOI: 10.1007/s00109-011-0741-7.
Peng, L. et al. (2011). Structure-activity study on a series of α-glutamic acid scaffold based compounds as new ADAMTS inhibitors. Bioorg Med Chem Lett 21, 4457.
     
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