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Antibodies  >  Primary Antibodies  >  Cell Cycle Control  >>  Anti-p53 (pSer15) Antibody polyclonal

Product Name Anti - p53 (pSer15) Antibody polyclonal
Size 25 g
Catalog # 28010-025
US$ $165
Description

Host: Rabbit

Species Reactivity: Human

Application: WB

Product Description: Rabbit polyclonal Anti-p53 (pSer 15) antibody is supplied as an epitope affinity purified rabbit IgG, 25 g in125 l of 1x PBS (pH 7.4) containing 0.05% sodium azide. This antibody was evaluated for specificity with a dot blot assay using synthetic p53 peptides. It only recognized the phosphorylated serine 15 of human p53, not other phosphorylated sites or non-phosphorylated p53.An antibody raised against the non-phosphorylated version of the sequence is available Rabbit polyclonal Anti-p53 (Ser) antibody, Cat# 28011.

Immunogen: A synthetic peptide corresponding to human p53 surrounding the phosphorylated site of Serine 15 (PPLpSQET) was used as the immunogen.

Application Notes: The following concentration ranges are recommended starting points. Optimal working concentrations for specific application should be determined by the investigator.

ELISA for immunizing peptide: 1:5,000-20,000
Western blot:1: 500-2,000
IP (not tested): 3.0-5.0 g/extract from 107 cells
Figure 1. Western blot of phosphorylated human p53 in hydroxyrea treated Cos-7 cell lysate probed with the Anti-p53 (pSer15) antibody (Cat# 28010). An immunoreactive band was detected at 53 kDa in the lysate.
Images Anti-p53 (pSer15) Antibody <i>polyclonal</i>
Detailed Information Datasheet
Storage 4C
Product Citations Suzuki, S. et al. (2003). Morphine suppresses lymphocyte apoptosis by blocking p53-mediated death signaling. Biochem Biophys Res Commun 308, 802.
     
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