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Assay Kits  >  Calpain Assay Kits  >>  SensoLyte® AMC Calpain Activity Assay Kit *Fluorimetric*

Product Name SensoLyte® AMC Calpain Activity Assay Kit *Fluorimetric*
Size 1 kit
Catalog # 72150
US$ $473
Description

The calpains are a family of intracellular Ca2+ supdependent cysteine proteases. The best-characterized calpains are isoforms 1 (m) and 2 (m). Calpains respond to Ca2+ signals by cleaving many specific proteins, thereby irreversibly modifying their function(s). They are implicated in a variety of Ca2+ regulated cellular processes as well as various pathological phenomena, such as ischemic injury, muscular dystrophy, diabetes, cataract, atherosclerosis, Alzheimer’s disease, and cancer. Calpains represent potential therapeutic targets for drug discovery. The SensoLyte® AMC Calpain Assay Kit is optimized for detecting calpain activities. This kit contains a fluorogenic peptide substrate, suc-LLVY-AMC. Upon calpain protease cleavage, suc-LLVY-AMC generates the AMC fluorophore emitting bright blue fluorescence and can be monitored at excitation/emission=354/442 nm. Increase in AMC fluorescence is proportional to calpain activity. The assay can detect both calpain 1 (μ) and 2 (m) activities and is ideal for kinetic study of these enzymes. The assays are performed in a convenient 96-well microplate format.

Kit size: 100 assays.

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Poster
Product Citations Lai, YS. et al. (2011). Calpain mediates epithelial cell microvillar effacement by enterohemorrhagic Escherichia coli.Front Microbiol 2, 222. doi: 10.3389/fmicb.2011.00222.
Wadosky, KM. et al. (2009). Regulation of the calpain and ubiquitin proteasome systems in a canine model of muscular dystrophy. Muscle & Nerve DOI: 10.1002/mus.22125.
Gallery, M. et al. (2007). The JAMM motif of human deubiquitinase Poh1 is essential for cell viability. Mol Cancer Ther 6, 262.
     
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