This peptide is histone H3 (1-21) with asymmetrical dimethylation at Arg2 and phosphorylation at Thr3, followed by a C-terminal GGK with biotinylation on the side chain of Lys. Asymmetrical dimethylation at Arg2 inhibits Lys 4 methylation and is associated with silenced chromatin. Phosphorylation of Thr3 begins in mitotic prophase and reverses in anaphase. This process is carried out by haspin in vitro. Provided at >95% peptide purity, this peptide was dissolved in distilled water at 1 mg/ml and re-lyophilized to powder form.
Klose, R. and Y. Zhang.Nat Struct Mol Biol14, 1058 (2007); Dai, J. et al. Gene Dev19, 472 (2005).
Sequence (One-Letter Code)
Sequence (Three-Letter Code)
H - Ala - Arg(Me2a) - pThr - Lys - Gln - Thr - Ala - Arg - Lys - Ser - Thr - Gly - Gly - Lys - Ala - Pro - Arg - Lys - Gln - Leu - Ala - Gly - Gly - Lys(Biotin) - OH