Immunogen: Rabbit polyclonal anti-p53 (Ser15) antibody was raised against a synthetic peptide (PPLSQET) near the N-terminus of human p53.
Application Notes: The following concentration ranges are recommended starting points. Optimal working concentrations for specific applications should be determined by the investigator.
ELISA for immunizing peptide: 1: 5,000-20,000 WB: 1: 500-2,000 Immunoprecipitation (not tested): 3.0-5.0 µg/extract from 107 cells
Figure 1. Western blot of p53 in MCF-7 (Lane A), Lovo (B), and HeLa (C) whole cell lysates labeled with rabbit polyclonal Anti-p53 (Ser15) antibody (Cat# 28011). Immunoreactive bands were detected at ~53 kDa in the lysates. Goat anti-rabbit IgG (H+L), highly cross-adsorbed, HiLyte Fluor™ 750-labeled secondary antibody (Cat# 61056-05-H750 or 61056-1-H750) was used and the signals detected using an infrared-imaging system (Odyssey, Li-Cor).