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Assay Kits  >  Amyloid Degrading Proteases Assay Kits  >>  SensoLyte 520 IDE Activity Assay Kit *Fluorimetric*

Product Name SensoLyte 520 IDE Activity Assay Kit *Fluorimetric*  NEW
Size 1 kit
Catalog # AS-72231
US$ $501
Description

Insulin degrading enzyme (IDE) is considered a physiological and pathological relevant enzyme. Growing evidence has indicated that IDE may be involved in the pathogenesis of Alzheimers disease and diabetes mellitus type 2.

The SensoLyte 520 IDE Activity Assay Kit can be used to detect enzyme activity in biological samples or in purified enzyme preparations. The unique FRET substrate was derived from an APP sequence designed to reduce cross reactivity with Neprilysin, ADAM10, TACE, BACE-1, and BACE-2. When active IDE cleaves the FRET substrate, it results in an increase of 5-FAM fluorescence, which is monitored at excitation/emission = 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is also less interfered by the autofluorescence of components in biological samples and test compounds. This assay can detect as low as 0.8 ng/mL active IDE.

The kit contains:
5-FAM/QXL520 IDE substrate, Ex/Em=490/520 nm upon cleavage
Assay buffer
Recombinant human IDE
Inhibitor
Fluorescence reference standard for calibration
A detailed protocol

Kit size: 100 assays (96-well plate)

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Figure 1. Inhibition of IDE activity by 1, 10-Phenanthroline as measured with SensoLyte 520 IDE Activity Assay Kit.
Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
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