β-Amyloid (1-40) & (1-42), HFIP-treated


AnaSpec, EGT Group is pleased to provide a whole suite of reagents for the advancement of Alzheimer’s disease (AD) research. From peptides to assay kits to antibodies to dyes, AnaSpec is the trusted source of integrated proteomics solutions for AD research. Amongst the more than 300 β-amyloid peptides, we offer β-amyloid (1-40) and (1-42) that are HFIP treated.

β-amyloids are amphiphilic peptides with a hydrophilic N-terminal domain (residues 1 to 28) and a hydrophobic C-terminal (residues 29 to 40-42), the latter corresponding to a part of the transmembrane domain of APP.1 β-Amyloid assembly into fibrils is initiated by a conformational transition from random coil to β-sheet (hence the name β-amyloid) and a nucleation-dependent aggregation process.1 Aβ peptides that are 39 to 42 amino acid residues in length with a molecular mass of approximately 4 kDa are the core components of neuritic plaques seen in Alzheimer’s disease (AD) brains. Studies have found that dissolving in HFIP favors alpha helical structure and tends to be monomeric.2 HFIP treatment of lyophilized Aβ-peptide results in a dense, homogeneous preparation of unaggregated peptide and samples can be stored as peptide films.3

Figure 1. A transmission electron micrograph (TEM) image of fibrils from β-amyloid (1-42), HFIP treated peptide (Cat# 64129).

Product

Size

Catalog #

Beta-Amyloid (1-40) • HFIP
DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV

0.5 mg

1 mg

64128-05

64128-1

Beta-Amyloid (1-42) • HFIP
DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA

0.5 mg

1 mg

64129-05

64129-1

Related Products

Peptides
Beta-Amyloid (1-28) and Related Peptides
Beta-Amyloid (1-40) and Related Peptides
Beta-Amyloid (1-42) and Related Peptides
Beta-Amyloid (1-43) to (1-49) and (1-55) Peptides
Beta-Amyloid Peptide Fragments
Beta-Amyloid Peptides - Mouse/Rat
Amyloid Toxicity Inhibitor Peptides
Amyloid Precursor Protein (APP) / Amyloid Precursor-like Protein (APLP)
Collagen-like Alzheimer Amyloid Plaque Component Precursor (CLAC-P)
Islet Amyloid Polypeptides (IAPP)
Humanins
Other Amyloid Related Peptides

Antibodies
Anti-β-Amyloid (1-40) & (1-42) mAbs
Anti-Tau, phospho & non-phospho

Assay Kits
SensoLyte® b-Amyloid (1-40) or (1-42) ELISA Assay Kits
SensoLyte® a-Secretase (TACE) Assay Kits
SensoLyte® Calpain Assay Kits
SensoLyte® Cathepsin B Assay Kits
SensoLyte® Cathepsin D Assay Kits

References

1.     Jarrett, JT. et al. Biochem 32, 4693 (1993).
2.     Barrow, CL. et al. J Mol Bio 225, 1075 (1992).
3.     Stine, WB. et al. J. Biol. Chem., 278, 11612 (2003).