SensoLyte« ThT Aβ42 & Aβ40 Aggregation kits - NEW


SensoLyte« ThT A▀40 and A▀42 Aggregation kits, using the fluorescent dye Thioflavin T (ThT), provide a convenient and standard method to measure A▀40 and A▀42 aggregation, respectively. In each of the kits, A▀40 or A▀42 peptide is pretreated to ensure it is in a monomeric state. Included in the kits is an optimized fibrillation buffer and two known inhibitors as controls. The assay is based on the property of ThT dye in which fluorescence (Ex/Em=440/484 nm) increase is proportional to the amount of dye bound to aggregated A▀ peptides.

Alzheimer's disease (AD), the most common cause of dementia, is characterized by the presence of senile plaques and neurofibrillary tangles, surrounded by damaged neurons. Beta-Amyloid (A▀) peptides, A▀40 (1-40) and A▀42 (1-42), were found to be a major component of the above plaques. Many studies suggest that these peptides can form toxic oligomers and fibrils under physiological conditions and rapidly aggregate. Since A▀ aggregation is evidently an essential event in the pathogenesis of AD, a reliable assay is important to study A▀ fibrillation kinetics and for screening A▀ aggregation inhibitors.


Product

Catalog #

SensoLyte« Thioflavin T ▀ - Amyloid (1 - 40) Aggregation Kit NEW

72213

SensoLyte« Thioflavin T ▀ - Amyloid (1 - 42) Aggregation Kit NEW

72214





Figure 1. Fluorescence signal is correlated with an increase of A▀40 fibril formation. Phenol Red, Morin, Rhodamine B, and Tannic Acid were added at 50 ÁM final concentration to inhibit A▀40 aggregation. Fluorescence was monitored at Ex/Em= 440/484 nm every 5 minutes at 37║C with 15 seconds shaking between reads (Flexstation II-384 fluorimeter, Molecular Devices).



Figure 2. Fluorescence signal is correlated with an increase of A▀42 fibril formation. Phenol Red, Morin, Tannic Acid, Dopamine, and o-Vanillin were added at 100ÁM final concentration to inhibit A▀42 aggregation. Fluorescence was monitored at Ex/Em= 440/484 nm every 5 minutes at 37║C with 15 seconds shaking between reads (Flexstation II-384 fluorimeter, Molecular Devices).



Figure 3. Thioflavin T staining of b-amyloid aggregates in an AD brain Image courtesy of Drs. J-P. Guo and Patrick L. McGeer (Kinsmen Laboratory of Neurological Research, University of British Columbia, Vancouver, Canada).

Related Products

Product

Catalog #

AggreSure ▀ - Amyloid (1 - 40), human NEW

72215

AggreSure ▀ - Amyloid (1 - 42), human NEW

72216

SensoLyte« Anti - a - Synuclein (Human, Mouse, Rat) ELISA Kit

55550

SensoLyte« Anti - Human ▀ - Amyloid (1 - 40) Quantitative ELISA *Colorimetric*

55551

SensoLyte« Anti - Human ▀ - Amyloid (1 - 42) Quantitative ELISA *Colorimetric*

55552

SensoLyte« Anti - Mouse/ Rat ▀ - Amyloid (1 - 40) Quantitative ELISA *Colorimetric*

55553

SensoLyte« Anti - Mouse/ Rat ▀ - Amyloid (1 - 42) Quantitative ELISA *Colorimetric*

55554

▀-Amyloid Peptides ľ Industryĺs largest collection

Anti-▀-Amyloid (1-40) & (1-42) mAbs

Anti-Tau antibodies, phospho & non-phospho ľ Wide selection


References
1. Hellstrand E., et. al., ACS Chem. Neurosci., (2010) 1: 13-18.
2. Karafin A., et. al., MEDIMOND, (2009) 11-15: 255-259.
3. Hudson S. A., et. al., FEBS J., (2009) 276: 5960-5972.
4. Liu R., et. al., Neurobiol. of Disease, (2005) 20: 74-81.
5. Kudva Y. C., et. al., Biochem. J., (1998) 331: 809-813.