Recombinant Proteins for Neuroscience Research


AnaSpec, EGT Group is pleased to supply recombinant proteins to complement our wide selection of Neuroscience related peptides, antibodies and assay kits. We offer Tau for Alzheimer's Disease (AD) research; α-Synuclein and DJ-1 proteins for Parkinson's Disease (PD) research; MOG (Myelin Oligodendrocyte Glycoprotein) and β-Synuclein for MS/EAE research. Additionally, α-Synuclein is available as endotoxin-free (EndoFree). α-Synuclein labeled with a green fluorescent dye (HiLyte™ Fluor 488, Ex/Em=490/520 nm) or a biotin is also available.

Alzheimer's Disease (AD) Research:

Tau-441

  • Tau is a microtubule-associated protein that stabilizes and promotes microtubule assembly.
  • In the human brain, it is present in six isoforms that are 352, 381, 383, 410, 412, and 441 amino acids in length (1-5). Tau-441 is the longest of the six Tau isoforms and contains 441 amino acids with molecular mass of 45.8 kDa.
  • When Tau is hyperphosphorylated, it can detach and form paired helical filament (PHF) aggregates (2-5).
  • PHFs were found in neurofibrillary tangles in Alzheimer's disease (AD) patients. As such, hyperphosphorylation of Tau has been linked to AD.
  • In addition to being modified with phosphate groups, Tau can also undergo glycosylation on the same regions where phosphorylation takes place. Discovery of this phenomenon has led to the hypothesis that glycosylation of Tau may help stabilize microtubule-Tau connection and prevent free Tau aggregation (5).
  • The sequence (Accession # AAC04279.1) corresponds to the full length human Tau-441 (2N4R isoform) and was expressed in E.coli.
  • AnaSpec provides the Tau-441 protein with or without a GST tag.


  • Tau-441 recombinant protein
    Fig. 1. Human Tau-441 on SDS-PAGE. Purified protein was loaded to a 10-20% Tris-HCl gel. CL=unpurified cell lysate, GST-Tau=purified GST-Tau fusion protein, Tau441=purified Tau without GST tag.
    Fig. 2. Western blot of in vitro phosphorylated Tau-441 protein with human GSK-3β. Purified Tau-441 was incubated with human GSK-3β. Phospho-Tau-441 (+GSK) & unphospho-Tau-441(-GSK) were resolved on a 10-20% Tris gel & transferred to nitrocellulose membrane. Proteins were probed with anti-Tau (393-411) and phosphospecific anti-Tau(pSer400) antibodies (AnaSpec Cat # AS-54979 & Cat# AS-54978, respectively).
    Tau-441 recombinant protein GST tagged
    Fig. 3. Human GST-Tau-441 on SDS-PAGE. Purified protein was loaded onto 10-20% Tris-HCl gel. CL=unpurified cell lysate, GST-Tau=purified GST-Tau fusion protein.

    Table 1. Tau-441 recombinant protein, with or without GST tag.

    Product

    Size

    Catalog #

    Recombinant Human Tau (Tau - 441) Protein NEW

    50 µg
    100 µg

    AS-55556-50
    AS-55556-100

    Recombinant Human Tau (Tau - 441) Protein, GST tagged NEW

    50 µg
    100 µg

    AS-55557-50
    AS-55557-100

Related Products:

Peptides

Antibodies

Assay Kits & Dyes

 

Parkinson's Disease (PD) Research:

alpha-Synuclein (1-140)

  • A major component of Lewy bodies in the affected neurons in Parkinson's disease (6-9).
  • This protein has a mass of 14.5 kDa (140 amino acids long) and consists of a conserved degenerative amino-terminal domain and an acidic carboxyl-terminal with higher sequence divergence (6-9).
  • Sequence (Accession# NP_000336) corresponds to the full length human alpha-synuclein protein without an affinity tag and was expressed in E. coli.
  • Each lot tested for aggregation
  • Available as EndoFree (less than 0.1 EU per μg/protein as tested by LAL method).

alpha_synuclein
Table 2. Listing of AnaSpec's alpha-Synuclein recombinant proteins.

Product
Size

Catalog #

EndoFree Recombinant Human alpha-Synuclein Protein NEW

100 μg
500 μg
1000 μg

AS-55555-100
AS-55555-500
AS-55555-1000

Recombinant Human alpha-Synuclein (1-140)

500 μg
1 mg

AS-55456-500
AS-55456-1000

Recombinant Human alpha-Synuclein (1-140), biotin labeled

200 μg

AS-55581

Recombinant Human alpha-Synuclein (1-140), HiLyte™ Fluor 488 labeled (Ex/Em=490/520 nm)

200 μg

AS-55457

Related Product:

DJ-1 (PARK7) Protein

  • Also known as PARK7 (Parkinson disease protein 7).
  • It is a 189-amino acid protein that is ubiquitously expressed in many organs including brain, liver, kidney, pancreas, heart, and others (10-14).
  • Although DJ-1 was originally discovered as a novel oncogene product, it was found to play several other roles in biological processes. This includes the regulation of RNA binding activity, fertility, anti-oxidative stress, and is linked to the early onset of Parkinson disease when mutated (10-14).
  • Sequence (Accession# NP_001116849) corresponds to the full length human DJ-1 protein ±N-terminal GST tag and was expressed in E. coli.

DJ-1

Table 3. Listing of AnaSpec's DJ-1 (PARK7) recombinant protein, with and without a GST tag.

Product
Size

Catalog #

DJ-1 (PARK7) Protein NEW

10 μg
100 μg
500 μg

AS-72219-10
AS-72219-100
AS-72219-500

DJ-1 (PARK7) Protein, Human, GST tagged (GST-DJ-1) NEW

10 μg
100 μg
500 μg

AS-72220-10
AS-72220-100
AS-72220-500

Related Product:

Multiple Sclerosis (MS) & EAE (Experimental autoimmune encephalomyelitis) Research:

MOG (1-125), Myelin Oligodendrocyte Glycoprotein (1-125)

  • A member of the immunoglobulin superfamily and is expressed exclusively in central nervous system (CNS).
  • Although MOG protein constitutes only 0.01-0.05% of the CNS myelin proteins, it was demonstrated that MOG protein is a crucial autoantigen for multiple sclerosis in humans and experimental autoimmune encephalomyelitis (EAE) in rodents and monkeys (15-19).
  • Human (Accession# CAQ10087), mouse (Accession# NP_034944) and rat (Accession# CAE84068) sequences were derived from the extracellular domain of MOG and were expressed in E. coli. These recombinant proteins contain a 6x His tag.
  • Tested for EAE induction

MOG
Table 4. Listing of AnaSpec's human, mouse and rat MOG (1-125) recombinant proteins.