|
|
| Beta-Secretase Assay Kits |
|
|
SensoLyte Beta-Secretase Assay Kit - This kit is discontinued.
As the world’s largest provider of amyloid peptides, AnaSpec has always been on the forefront in the development of novel solutions for
amyloid-related research. We are pleased to announce our latest breakthrough— the new SensoLyte™ 520 Beta-Secretase Assay Kit.
Beta-secretase catalyzes a key step in the production of beta-amyloid peptides seen accumulated in senile plagues of Alzheimer’s disease (AD)
brains. In order to facilitate high throughput screening of AD drug candidates, we have developed a new SensoLyte™ 520 beta-secretase assay
kit using a fluorescence resonance energy transfer (FRET) peptide, HiLyte Fluor™ 488-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(QXL™ 520)-OH
(see Figure 1). The sequence of this FRET peptide is derived from the beta-secretase cleavage site of beta-amyloid precursor protein(APP) with
Swedish mutation.6 This mutation enhances the susceptibility of APP to beta-secretase and results in an early onset of AD.
Figure 1. The absorption spectrum of QXL™520 overlaps with the emission spectrum of HiLyte Fluor™ 488. HiLyte Fluor™ 488 extinction
coefficient is 92,400M-1 cm-1.
This assay has good sensitivity (0.03 mU/ml) with a signal-to-background ratio >10 after a 30-minute incubation. This homogeneous assay can
be used to continuously monitor product formation. Validated with known inhibitors, the IC50 value for one of the inhibitors determined with
SensoLyte™ 520 beta-secretase assay kit was consistent with published data.
SensoLyte™ 520 Beta-Secretase Assay Kit
Click here to request additional literature
To view a Beta-Secretase Assay Kit poster presented at the 2007 Experimental Biology in Washington, DC, please click here.
|
|
|
|
|
|
|
|
|
|
|
|
|
