Recognizing the growing need for innovative tools for the study of apoptosis,
AnaSpec continues to expand its collection of fluorimetric apoptosis related
assay products. We are pleased to introduce our newest offering – the SensoLyte™
Homogeneous AFC Caspase-8 Assay Kit.
Apoptosis is programmed cell death which leads to the elimination
of damaged, unwanted, or unneeded cells without releasing harmful substances
into the surrounding area. Apoptosis is involved in a variety of physiological
and pathological events, 1 ranging from normal fetal development to diseases, such as cancer, 2 organ failure and neurodegenerative diseases. Caspases are proteases which play
essential roles in apoptosis and inflammation. There are two types of apoptotic
caspases: initiator caspases and effector caspases. Caspase-8, an initiator
enzyme, also known as Mch5, MACH and FLICE, accepts signals from death receptors
(FAS, TRAIL, TNF) and activates downstream effector caspases through proteolytic
cleavage. 3,4 Activated effector caspases induce apoptosis through cleavage of other cellular
substrates such as PARP and DFF.
The SensoLyte™ Homogeneous AFC Caspase-8 Assay Kit uses Ac-IETD-AFC as the
fluorogenic indicator to measure caspase-8 activity. Upon cleavage of the
substrate by caspase-8, free AFC (7-amido-4-trifluoromethylcoumarin) emits
a yellow-green fluorescence, which can be quantified at excitation/emission=380nm/500nm.
A bi-functional assay buffer in this kit is optimized for cell lysis and measurement
of the enzyme activity. This kit can assay caspase-8 activity in cells grown
in a 96-well plate or in larger plates or flasks. The kit is adaptable for
high throughput screening of apoptosis inducers and inhibitors.
- Fluorimetric readout (Ex/Em=380nm/500nm)
- Adaptable for HTS
- Homogeneous assay
Caspase Assay Kits
Related Peptides & Substrates
1. Thornberry, NA. and Y. Lazebnik, Science 281, 1312 (1998).
2. Reed, JC. J. Clin. Oncol 17, 2941 (1999).
3. Muzio, M. et al. J. Biol. Chem 272, 2952 (1997).
4. Takahashi, A. et al. Oncogene 14, 2741 (1997).