Fluorescently Labeled GST & 6xHis Tag Antibodies – Spanning the Visible Spectrum


Different gene expression systems have been used to produce recombinant proteins.1-2 Protein tags are peptide sequences genetically introduced onto a recombinant protein. Tags include short peptides(FLAG, c-myc, 6X-His) or proteins (glutathione-s-transferase - GST). This tagging technique aids in the characterization, localization or purification of the recombinant expressed protein.3 Antibodies raised against fusion-tags are useful in biochemical or immunohistochemistry applications.

AnaSpec is pleased to announce the availability of fluorescently labeled GST and 6X-His antibodies. These antibodies are labeled with a selection of dyes, including our proprietary HiLyte™ Fluor and HiLytePlus™ dyes, which span the visible spectrum. This gives researchers a wide range of wavelength choices for use in immunohistochemistry or immunofluorescence applications. Non-labeled or biotin labeled versions are also available.

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Polyclonal Anti-6xHis Tag

Product

Ex/Em

Catalog #

Polyclonal Anti-His-tag NEW

N/A

29673

Polyclonal Anti - His Tag IgG, FITC labeled NEW

495/520

29673-FITC

Polyclonal Anti - His Tag IgG, HiLyte™ Fluor 488 labeled NEW

499/523

29673-H488

Polyclonal Anti - His Tag IgG, HiLyte™ Fluor 555 labeled NEW

499/523

29673-H555

Polyclonal Anti - His Tag IgG, HiLyte™ Fluor 647 labeled NEW

653/673

29673-H647

Polyclonal Anti - His Tag IgG, Biotin labeled NEW

N/A

29673-BIOT


Monoclonal Anti-6xHis Tag

Product

Ex/Em

Catalog #

Monoclonal Anti-His Tag NEW

N/A

61250

Monoclonal Anti - His Tag IgG, AMCA labeled NEW

344/446

61250-AMCA

Monoclonal Anti - His Tag IgG, Biotin labeled NEW

N/A

61250-BIOT

Monoclonal Anti - His Tag IgG, FAM labeled NEW

495/520

61250-FAM

Monoclonal Anti - His Tag IgG, FITC labeled NEW

495/520

61250-FITC

Monoclonal Anti - His Tag IgG, HiLyte™ Fluor 488 labeled NEW

499/523

61250-H488

Monoclonal Anti - His Tag IgG, HiLyte™ Fluor 555 labeled NEW

553/568

61250-H555

Monoclonal Anti - His Tag IgG, HiLyte™ Fluor 647 labeled NEW

653-673

61250-H647

Monoclonal Anti - His Tag IgG, HiLytePlus™ 555 labeled NEW

552/567

61250-Plus555

Monoclonal Anti - His Tag IgG, HiLytePlus™ 647 labeled NEW

649/669

61250-Plus647

Monoclonal Anti - His Tag IgG, ROX labeled NEW

573/602

61250-ROX

Monoclonal Anti - His Tag IgG, TAMRA labeled NEW

555/580

61250-TAMR


Monoclonal Anti-GST Tag

Product

Ex/Em

Catalog #

Monoclonal Anti - GST Tag NEW

N/A

29531

Monoclonal Anti - GST Tag AMCA labeled NEW

344/446

29531-AMCA

Monoclonal Anti - GST Tag FAM labeled NEW

495/520

29531-FAM

Monoclonal Anti - GST Tag FITC labeled NEW

495/520

29531-FITC

Monoclonal Anti - GST Tag HiLyte™ Fluor 488 labeled NEW

499/523

29531-H488

Monoclonal Anti - GST Tag HiLytePlus™ 555 labeled NEW

553/568

29531-Plus555

Monoclonal Anti - GST Tag HiLytePlus™ 647 labeled NEW

653-673

29531-Plus647

Monoclonal Anti - GST Tag TAMRA labeled NEW

555/580

29531-TAMR

Monoclonal Anti - GST Tag ROX labeled NEW

573/602

29531-ROX

Monoclonal Anti - GST Tag Biotin labeled NEW

N/A

29531-BIOT

Related Products and Services:

Anti-FLAG
Anti-HA’s
Anti-GFP’s
Anti-Calmodulin
Anti-Maltose Binding Protein
Custom labeling of antibodies
Tag Peptides

REFERENCES:
1. Zheng et al. (2003) J. Biochem. 133: 577-581.
2. Amrein et al. (1995) PNAS. 92:1048-1052.
3. Nakajima K. and Yaoita Y. (1997) Nucleic Acids Res. 25: 2231-2232.
4. Medina et al. (2000) Nucleic Acids Res. 28: i-vi.