Different gene expression systems have been used to produce recombinant proteins.^1-2  Protein tags are peptide sequences genetically introduced onto a recombinant protein. Tags include short peptides(FLAG, c-myc, 6X-His) or proteins (glutathione-s-transferase - GST).  This tagging technique aids in the characterization, localization or purification  of the recombinant expressed protein.³  Antibodies raised against fusion-tags are useful in biochemical or immunohistochemistry applications.   

AnaSpec is pleased to announce the availability of fluorescently labeled GST and 6X-His antibodies. These antibodies are labeled with a selection of dyes, including our proprietary HiLyte Fluor^TM and HiLytePlus^TM dyes, which span the visible spectrum.   This gives researchers a wide range of wavelength choices for use in immunohistochemistry or immunofluorescence applications. Non-labeled or biotin labeled versions are also available.

Request AnaSpec’s newest Antibodies brochure today.  Click here

Polyclonal Anti-6xHis Tag

Product	Ex/Em	Catalog #
Polyclonal Anti-His-tag NEW	N/A	29673
Polyclonal Anti - His Tag IgG, FITC labeled  NEW	495/520	29673-FITC
Polyclonal Anti - His Tag IgG, HiLyte Fluor™ 488 labeled  NEW	499/523	29673-H488
Polyclonal Anti - His Tag IgG, HiLyte Fluor™ 555 labeled  NEW	499/523	29673-H555
Polyclonal Anti - His Tag IgG, HiLyte Fluor™ 647 labeled  NEW	653/673	29673-H647
Polyclonal Anti - His Tag IgG, Biotin labeled  NEW	N/A	29673-BIOT

Monoclonal Anti-6xHis Tag

Product	Ex/Em	Catalog #
Monoclonal Anti-His Tag  NEW	N/A	61250
Monoclonal Anti - His Tag IgG, AMCA labeled  NEW	344/446	61250-AMCA
Monoclonal Anti - His Tag IgG, Biotin labeled  NEW	N/A	61250-BIOT
Monoclonal Anti - His Tag IgG, FAM labeled  NEW	495/520	61250-FAM
Monoclonal Anti - His Tag IgG, FITC labeled  NEW	495/520	61250-FITC
Monoclonal Anti - His Tag IgG, HiLyte Fluor™ 488 labeled  NEW	499/523	61250-H488
Monoclonal Anti - His Tag IgG, HiLyte Fluor™ 555 labeled  NEW	553/568	61250-H555
Monoclonal Anti - His Tag IgG, HiLyte Fluor™ 647 labeled  NEW	653-673	61250-H647
Monoclonal Anti - His Tag IgG, HiLytePlus™ 555 labeled  NEW	552/567	61250-Plus555
Monoclonal Anti - His Tag IgG, HiLytePlus™ 647 labeled  NEW	649/669	61250-Plus647
Monoclonal Anti - His Tag IgG, ROX labeled  NEW	573/602	61250-ROX
Monoclonal Anti - His Tag IgG, TAMRA labeled  NEW	555/580	61250-TAMR

Monoclonal Anti-GST Tag

Product	Ex/Em	Catalog #
Monoclonal Anti - GST Tag  NEW	N/A	29531
Monoclonal Anti - GST Tag AMCA labeled  NEW	344/446	29531-AMCA
Monoclonal Anti - GST Tag FAM labeled  NEW	495/520	29531-FAM
Monoclonal Anti - GST Tag FITC labeled  NEW	495/520	29531-FITC
Monoclonal Anti - GST Tag HiLyte Fluor™ 488 labeled  NEW	499/523	29531-H488
Monoclonal Anti - GST Tag HiLytePlus™ 555 labeled  NEW	553/568	29531-Plus555
Monoclonal Anti - GST Tag HiLytePlus™ 647 labeled  NEW	653-673	29531-Plus647
Monoclonal Anti - GST Tag TAMRA labeled  NEW	555/580	29531-TAMR
Monoclonal Anti - GST Tag ROX labeled  NEW	573/602	29531-ROX
Monoclonal Anti - GST Tag Biotin labeled  NEW	N/A	29531-BIOT

Related Products and Services:

Anti-FLAG
Anti-HA’s
Anti-GFP’s
Anti-Calmodulin
Anti-Maltose Binding Protein
Custom labeling of antibodies
Tag Peptides

REFERENCES:
1.    Zheng et al. (2003) J. Biochem. 133: 577-581.
2.    Amrein et al. (1995) PNAS. 92:1048-1052.
3.    Nakajima K.  and Yaoita Y.  (1997)  Nucleic Acids Res.  25: 2231-2232.
4.    Medina et al. (2000) Nucleic Acids Res. 28:  i-vi.