Anti-Keratin-18 (Asp237) and Anti-Keratin-19(Asp237) Novel Antibodies for Apoptosis Studies


AnaSpec is pleased to announce the release of two keratin antibodies that recognize both the intact and caspase digested N-terminal of K18 and K19 at Asp237. These antibodies are useful tools for studying keratin dynamics in mice undergoing hepatocyte apoptosis, patients with cirrhosis, and also in apoptotic cells of various epithelial human tumors in a non-invasive manner.

Intermediate filaments (IFs), together with actin microfilaments and tubulin microtubules, comprise the three major cytoskeletal networks that are found in most eukaryotic cells.1 Keratins, which are the largest IF protein subgroup, can be divided into the acidic type I (K9-K20) and basic type II (K1-K8) Keratins.2 Typically, epithelial cells express at least one of each type, and the proteins exist in the cells as noncovalent obligate heteropolymers. Keratin networks are highly dynamic and reorganized during cell differentiation, mitosis and apoptosis, and also play important cytoprotective and structural support roles for the cell.3,4 During apoptosis, the epithelial keratins 18 and 19 undergo caspase-mediated proteolysis, leading to the disassembly of the nuclear envelope and cytoplasmic IF networks.5 K18 and K19 undergo caspase-cleavage at the conserved Asp237 in their rod domains.5,6 In contrast to other type I keratins, K18 is unique in that it includes another caspase-cleavage site at Asp396 of the carboxy-terminal domain.7

Rabbit anti-human Keratin-18 (Asp237) or anti-human Keratin-19 (Asp237) polyclonal antibodies were raised against a synthetic peptide corresponding to an internal sequence surrounding amino acid 237 (GLTVEVD) of human Keratin-18 or amino acid 237 (QVSVEVD) of human Keratin-19, respectively. Anti-Keratin 18 reacts to both cleaved keratin-18 (Asp237) (29 kDa) and intact Keratin-18 (49 kDa) proteins from anisomycin-treated human colonic carcinoma HT29 cell lysate and Fas antibody-induced mouse liver lysate (see figure below).6 This antibody also reacts to cleaved Keratin-19 (Asp237) (29 kDa).6 Anti-Keratin 19 reacts to both cleaved keratin-19 (Asp237) (29 kDa) and intact Keratin-19 (45 kDa) proteins from anisomycin-treated human colonic carcinoma HT29 cell lysate.6 This antibody also reacts to cleaved Keratin-18 (Asp237) (29 kDa).6 Both antibodies are human, mouse, and monkey reactive.

Product

Size

Catalog #

Anti-Keratin-18 (Asp237); human NEW

250 L

55148A

Anti-Keratin-19 (Asp237); human NEW

250 L

55149A


Anti-K-18 (asp237) Anti-K-19 (asp237)

Click here to request AnaSpecs latest Antibodies brochure

Click here to view the abstract of a paper on anti-K18 and K-19 that recognize only the cleaved K18/K19 Asp 237 antibodies published by Stanford University and AnaSpec

Related Products:

Antibodies

Size

Catalog #

Anti-human Keratin-18 (IN-2)

50 g

54369

Anti-human Keratin-19 (IN)

50 g

54370

Anti-Keratin-7

50 g

53607

Anti-Keratin-8

50 g

53608

Anti-Keratin-10

50 g

53604

Anti-Keratin-14

50 g

53605

Anti-Keratin-20

50 g

53606

Anti-Keratin; LMW

50 g

53610

Anti-Keratin; HMW

50 g

53609


Peptide

Size

Catalog #

Keratin K18-C
RPVSSAApSVYAGAC

1 mg

5 mg

60214-1

60214-5

References:

1. Ku, NO. et al. Am J. Physiol. 277, G1108 (1999).
2. Schweizer, J. et al. J. Cell Biol. 174, 169 (2006).
3. Omary, MB. et al. Trends Biochem. Sci. 31, 383 (2006).
4. Omary, MB. et al. N. Engl. J. Med. 351, 2087 (2004)
5. Ku, NO. et al. J. Biol. Chem. 272, 33197 (1997).
6. Tao, GZ. et al. J. Pathology. 215, 164 (2008).
7. Ku, NO. and Omary, MB. J. Biol. Chem. 276, 26792 (2001).