As advances in kinase research continue to speed up, researchers are constantly in search of tools that can keep up. With over 300 research-ready kinase/phosphatase substrates, AnaSpec makes sure that your kinase discovery is ready to go.

Protein kinases are a transferase family of self-regulatory enzymes that catalyze the phosphorylation of other proteins by covalently attaching phosphate groups to them, using ATP as a phosphate donor. Reversible protein phosphorylation-dephosphorylation has a principal role in the regulation of essentially all cellular functions and most aspects of cell life, and phosphorylation by protein kinases is a major signal transduction mechanism that regulates gene expression, proliferation, membrane transport, cell metabolism and many other cellular functions by modifiying other proteins.

This month we are pleased to showcase our extensive collection of kinase/phosphatase substrates. These substrates can be found grouped according to their kinase families. A single substrate can have a dizzying number of modifications (see Table 1).

Research moves fast. Kinase/phosphatase substrates from AnaSpec are GO™.

To get started finding the kinase/phosphatase substrate you need, just type in the sequence you are looking for into our Smart Search tool.

For peptide array needs, please email your requirements to service@anaspec.com.

Kinase Substrate Categories

AKT/PKB/Rac-protein kinases

Calcineurin (PP2B)

CaMK (Ca2+/calmodulin-dependent protein kinase)

CaMK II (Ca2+/calmodulin-dependent protein kinase II)

CDC2; CDK1 (Cyclin-dependent protein kinase 1)

CDK5 (Cyclin-dependent Kinase 5)

CK I (Casein Kinase I)

CK II (Casein Kinase II)

EGFR (Epidermal Growth Factor Receptor)

Glycogen Synthase

GSK-3 (Glycogen Synthase Kinase - 3)

GSK-3β (Glycogen Synthase Kinase - 3β)

Insulin Receptor Tyrosine Kinase

Kinase Substrate Library

MAP kinases

Myristoylated Alanine-rich C-Kinase

P60c-src

PKA (Protein Kinase A)

PKC (Protein Kinase C)

PKG (Protein Kinase G)

Protein Tyrosine Kinases

PTP (Protein Tyrosine Phosphatase)

PTP 1B (Protein Tyrosine Phosphatase 1B)

S6 Kinases

Tyrosine Hydroxylase

Tyrosine Kinase Peptide 1

Tyrosine Kinase Peptide 3

Table 1. Modifications of Kemptide (Leu-Arg-Arg-Ala-Ser-Leu-Gly)

LRRASLG	Native	NA	771.9	1 mg 5 mg 25 mg	22593 22594 22595
5-FAM-LRRASLG	Green fluorescent	494/522	1130.2	1 mg 5 mg	29933-1 29933-5
5-TMR-LRRASLG	Red fluorescent	546/574	1184.4	1 mg 5 mg	29934-1 29934-5
Biotin-LRRASLG	Biotinylated	NA	998.2	1 mg 5 mg 25 mg	60527 24533 24534
Biotin-LC-LRRASLG	Biotinylated-LC	NA	1110.4	5 mg 25 mg	24535 24536
HiLyte FluorTM 488-LRRASLG	Green fluorescent	498/525	1128.3	1 mg	61233
LRRASVA	[Val6, Ala7] Kemptide	NA	771.9	5 mg 25 mg	22596 22597
LRRWSLG	[Trp4] Kemptide	NA	887.1	5 mg 25 mg	22598 22599
LRRAALG	Negative Control	NA	755.9	1 mg 5 mg	24428 24429
LKRA-pY-LG-NH2	[Lys2, pTyr5] Kemptide	NA	899.0	1 mg 5 mg	20290 24528
LKRA-pT-LG	[Lys2, pThr5] Kemptide	NA	837.0	1 mg 5 mg	20295 24497
LRRA-pS-LG	Phosphorylated	NA	851.9	1 mg 5 mg	60528-1 60528-5
Biotin-LRRA-pS-LG-NH2	Biotinylated, Amidated	NA	1077.2	1 mg 5 mg	60565-1 60565-5
Biotin-LC-LRRA-pS-LG	Biotinylated with LC	NA	1191.4	1 mg 5 mg	60566-1 60566-5
Biotin-LRRA-pS-LG	Biotinylated	NA	1078.2	1 mg 5 mg	60567-1 60567-5
HiLyte FluorTM 488- LRRA-pS-LG	Green fluorescent	498/525	1208.3	1 mg	61234
5-TMR-LRRA-pS-LG	Red fluorescent	NA	1264.4	1 mg	60467-1