IRAK Antibodies pThr 209 & 387 NEW


AnaSpec, a leader in phosphopeptide synthesis, is proud to introduce two phosphospecific IRAK1 polyclonal antibodies anti-IRAK1 (pThr 209) and anti-IRAK1 (pThr387). As seen on the dot blots (Figures 1-2), these antibodies are specific for the phosphothreonine sequence and not the non-phosphothreonine sequence. As well, the cross-reactivity between the pThr209 and pThr 387 antibodies is minimal (Figure 3).

Interleukin-1 (IL-1) receptor-associated kinase (IRAK) is a serine/threonine-specific kinase (1). The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK3/IRAK-M and IRAK4). The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1RI, AcP, MyD88 and IRAKs (2). IRAK undergoes autophosphorylation shortly after IL-1 stimulation and serves as a signaling molecule to mediate IL-1 response (3). IRAK mediates a signaling cascade leading to NF-KB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF) (1, 4).

Rabbit anti-IRAK-1(pThr209) cat# 55331 polyclonal antibody was raised against a peptide corresponding to human IRAK1 at the phosphorylated threonine 209 (EISRGpTH), while Rabbit anti-IRAK-1(pThr387) cat# 55333 polyclonal antibody corresponds to human IRAK1 at the phosphorylated threonine 387 (VRG-pTLAY). The non-phosphorylated version of these antibodies is also available. The IRAK1 antibodies have been evaluated for specificity by dot blot.

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Figure 1. Dot blot of anti-IRAK1 (pThr209) and anti-IRAK1 (paired pThr209 non-phosphorylated).
Figure 2. Dot blot of anti-IRAK1 (pThr387) and anti-IRAK1 (paired pThr387 non-phosphorylated).
Figure 3. Dot blot of anti-IRAK1 (pThr209) and anti-IRAK1 (paired pThr209 non-phosphorylated) and anti-IRAK1 (pThr387) and anti-IRAK1 (paired pThr387 non-phosphorylated).


Product Size Cat #
AAnti-IRAK1 (pThr 209) NEW

50 g

55331

Anti-IRAK1 (pThr 387) NEW

50 g

55333

Anti-IRAK1 (paired 209) NEW

50 g

55332

Anti-IRAK1 (paired 387) NEW

50 g

55334

Related Products:

Other IRAK antibodies

>300 Kinase & Phosphatase Peptide Substrates

References:

1. Dinarello, CA. Blood 87, 2095-2147 (1996).
2. Takaesu, G. et al. Mol. Cell. Biol. 21, 2475-2484 (2001).
3. Jianing H. et al. Proc. Natl. Acad. Sci. USA 94, 12829 (1997).
4. Robinson D. et al. Immunity 7, 571 (1997).

AnaSpec is pleased to introduce new IRAK1 phosphothreonine 209 and 387 antibodies.