AnaSpec is pleased to announce the release of a long wavelength
assay for Lysine-Specific Demethylase –1 (LSD1), the SensoLyte®
LSD1 Assay Kit, Ex/Em=530/590 nm.
LSD1 is a histone-modifying enzyme catalyzing lysine demethylation
of histone H3. It plays an important role in regulation of gene expression,
acting either as a transcription co-repressor or co-activator.1
The enzyme is implicated in cellular
differentiation and has been considered as a potential target for drug discovery.2
LSD1 assay kit provides a convenient assay for screening of LSD1 inhibitors
and for detection of LSD1 activity. This assay consists of two steps. In the
first step, LSD1 enzyme is incubated with a methylated peptide substrate and
hydrogen peroxide (H2O2) is generated. In the second step,
in the presence of peroxidase, H2O2 reacts with fluorogenic
substrate to produce fluorescence signal, which is
monitored at Ex/Em=530/590 nm.
Figure 1. Inhibition of LSD1 activity by Tranylcypromine (an LSD1 inhibitor)
was measured using the SensoLyte™ LSD1 assay kit.
Related Products and Services
Peptides – includes a number of recently released histone peptides
HDAC Assay Kits
Custom Peptide Synthesis Service
Custom Assay Development
- Culhane, J. C. et al. Curr
Opin Chem Biol. 11, 561 (2007).
- Shi, Y. Nat. Rev. Genet. 8, 829 (2007).