AnaSpec is pleased to announce the release of a long wavelength assay for Lysine-Specific Demethylase –1 (LSD1), the SensoLyte® LSD1 Assay Kit, Ex/Em=530/590 nm.

LSD1 is a histone-modifying enzyme catalyzing lysine demethylation of histone H3. It plays an important role in regulation of gene expression, acting either as a transcription co-repressor or co-activator.¹ The enzyme is implicated in cellular differentiation and has been considered as a potential target for drug discovery.²  

The SensoLyte® LSD1 assay kit provides a convenient assay for screening of LSD1 inhibitors and for detection of LSD1 activity. This assay consists of two steps. In the first step, LSD1 enzyme is incubated with a methylated peptide substrate and hydrogen peroxide (H₂O₂) is generated. In the second step, in the presence of peroxidase, H₂O₂ reacts with fluorogenic substrate to produce fluorescence signal, which is monitored at Ex/Em=530/590 nm.

Figure 1.  Inhibition of LSD1 activity by Tranylcypromine (an LSD1 inhibitor) was measured using the SensoLyte™ LSD1 assay kit.

Related Products and Services

Histone Peptides – includes a number of recently released histone peptides
SensoLyte® HDAC Assay Kits
Custom Peptide Synthesis Service
Custom Assay Development

References

1. Culhane, J. C. et al. Curr Opin Chem Biol. 11, 561 (2007).
2. Shi, Y. Nat. Rev. Genet. 8, 829 (2007).