AnaSpec, the maker of the industry’s first and only colorimetric anti-MOG assay kits, is pleased to announce the release of two multiple sclerosis (MS)/experimental autoimmune encephalomyelitis (EAE) related ELISA assays kits, the SensoLyte^® Anti-PLP(139-151) Assay Kit and the SensoLyte^® Anti-PLP(178-191) Assay Kit. These kits, precoated either with PLP (139-151) or PLP (178-191) peptide are optimized to detect mouse anti- PLP (139-151) or anti-PLP (178-191) IgG, respectively. The amount of IgG antibodies in mouse serum or cerebrospinal fluid is quantified using ELISA. Ample materials and reagents are provided to perform 80 assays in a 96-well plate format.

• Convenient Format
— Pre-coated and pre-blocked 96-well strip plate
— Ready-to-use substrate solution and other assay components
— 2-3 hours assay time at room temperature
• Minimal Sample Size
— Requires only 0.5-1 μl of serum or cerebrospinal fluid to perform assay
• High Sensitivity
— Detects as low as 100 pg of anti-PLP (139-151) or anti-PLP (178-191) IgG
— No cross-reactivity between two kits
• Broad Dynamic Range
— 8-500 ng antibody/ml serum (depending on colorimetric developing time)

Proteolipid protein (PLP) is the most abundant protein in the central nervous system (CNS) myelin sheath and is highly conserved among species.^1-2 PLP has a 50% hydrophobic amino acids content and a molecular weight of 30 kDa.¹ Mouse PLP (139-151) and PLP (178-191) are able to induce autoantibody production and relapsing-remitting neurological disease causing extensive plaque-like demyelination.^1-4 Autoantibody response to mouse PLP (139-151) and PLP (178-191) has been observed in induced experimental autoimmune encephalomyelitis (EAE) in B10.PL, C57BL/6, BALB/c, SJL, F1, and SWR mice strains.^3-4 However, the exact pathological role and action of anti-mouse PLP (139-151) and anti-PLP (178-191) autoantibody is not well understood.^1-4

Figure 1. The standard curve for anti-mouse PLP (139-151) on the left and anti-mouse PLP (178-191) on the right.

Anti-PLP (139-151), rabbit polyclonal antibody
Anti-PLP (178-191), rabbit polyclonal antibody
MOG Proteins, Peptides, Antibodies & Assay Kits
MBP Peptides
PLP Peptides

View a poster on Suppressive Role of CD72 in Experimental Autoimmune Encephalomyelitis

1 Tzakos, AG. et al. Curr. Med. Chem.12, 1569 (2005).
2 Miller, D. et al. Curr. Protocols Immunol .15.1.1.(2007).
3 Tuohy, V. et al. J. Neuroimmunol.. 39,67 (2003).
4 Kuchroo, V. et al. Pathobio. 59, 305 (1991).