AnaSpec, Eurogentec’s newest North American division, is pleased to offer SIRT1 assay kits in the green range (Ex/Em=490/520 nm). These kits, the SensoLyte^® 520 FRET SIRT1 Assay Kit and SensoLyte^® Green SIRT1 Assay Kit , represent the industry’ first and longest wavelength SIRT 1 assays. These two-step, homogeneous assays uses a FRET substrate with 5-FAM/QXL™ 520 as the FRET pair in the former and a green fluorophore containing substrate in the latter. The long wavelength fluorescence of the green dyes employed in these two kits is less interfered by the autofluorescence of cell components and test compounds; thus making these kits ultra-sensitive. Figure 1 shows a better signal to background ratio of the SensoLyte^® Green SIRT1 Assay Kit compared to a leading competitor’s blue range SIRT 1 assay.

Figure 1. SensoLyte® Green SIRT 1 Assay Kit shows better signal to background ratio compared with a leading competitor’s blue range SIRT1 assay kit.

Histone deacetylases (HDACs) act as transcriptional repressors of genes catalyzing the removal of acetyl groups from a ε-N-acetyl lysine of histone.¹ Sirtuins comprise a unique class of nicotinamide adenine dinucleotide (NAD+)-dependent deacetylases (class III HDACs) that target multiple protein substrates to execute diverse biological functions. Sirtuins catalyze a reaction that couples lysine deacetylation to NAD hydrolysis, yielding O-acetyl-ADP-ribose and nicotinamide.²

Sirtuin 1 (SIRT1), the human homolog of yeast Sir2 (Silent Information Regulator 2), is the most studied of the seven members of sirtuin family. SIRT1 have been implicated in several important cellular processes, including genomic stability and DNA repair,^3,4 p53-mediated apoptosis,⁵ adipogenesis,⁶ and aging.^{7, 8}

Related Products

SensoLyte® HDAC Assay Kits
SensoLyte® LSD1 Assay Kit
Histone H3 Peptides
Full Listing of Histone Peptides
Histone Related Antibodies

References:

1. Sterner, DE. et al. Microbiol. Mol. Biol. Rev. 64, 435 (2000).
2. Longo, V and Kennedy, B. Cell 126, 257 (2006).
3. Yamagata, K and Kitabayashi, I. Biochem. Biophys. Res. Commun. 390, 1355 (2009).
4. Wang, RH. et al. Cancer Cell 14, 312 (2008).
5. Vaziri, H. et al. Cell 107, 149 (2001).
6. Picard, F. et al. Nature 429, 771 (2004).
7. Cohen, HY. et al. Science 305, 390 (2004).
8. Trapp, J. and Jung, M. Curr. Drug Target 7, 1553 (2006).