AnaSpec, Eurogentec’s newest North American division, is pleased to offer SIRT1
assay kits in the green range (Ex/Em=490/520 nm). These kits,
520 FRET SIRT1 Assay Kit and SensoLyte®
Green SIRT1 Assay Kit , represent the industry’ first and longest wavelength
SIRT 1 assays. These two-step, homogeneous assays use
a FRET substrate with 5-FAM/QXL™ 520 as the FRET pair in the former and a green
fluorophore containing substrate in the latter. The long wavelength fluorescence
of the green dyes employed in these two kits is less interfered by the autofluorescence
of cell components and test compounds; thus making these kits ultra-sensitive.
Figure 1 shows a better signal to background ratio of the SensoLyte®
Green SIRT1 Assay Kit compared to a leading competitor’s blue range SIRT
||Figure 1. SensoLyte®
Green SIRT 1 Assay Kit shows better signal to background ratio compared
with a leading competitor’s blue range SIRT1 assay kit.
Histone deacetylases (HDACs) act as transcriptional repressors of genes catalyzing
the removal of acetyl groups from a ε-N-acetyl lysine of histone.1
Sirtuins comprise a unique class of nicotinamide adenine dinucleotide (NAD+)-dependent
deacetylases (class III HDACs) that target multiple protein substrates to execute
diverse biological functions. Sirtuins catalyze a reaction that couples lysine
deacetylation to NAD hydrolysis, yielding O-acetyl-ADP-ribose and nicotinamide.2
Sirtuin 1 (SIRT1), the human homolog of yeast Sir2 (Silent Information Regulator
2), is the most studied of the seven members of sirtuin family. SIRT1 have been
implicated in several important cellular processes, including genomic stability
and DNA repair,3,4 p53-mediated apoptosis,5 adipogenesis,6
and aging.7, 8
HDAC Assay Kits
LSD1 Assay Kit
of Histone Peptides
1. Sterner, DE. et al. Microbiol. Mol. Biol. Rev. 64,
2. Longo, V and Kennedy, B. Cell 126, 257 (2006).
3. Yamagata, K and Kitabayashi, I. Biochem. Biophys. Res. Commun. 390,
4. Wang, RH. et al. Cancer Cell 14, 312 (2008).
5. Vaziri, H. et al. Cell 107, 149 (2001).
6. Picard, F. et al. Nature 429, 771 (2004).
7. Cohen, HY. et al. Science 305, 390 (2004).
8. Trapp, J. and Jung, M. Curr. Drug Target 7, 1553 (2006).