Custom Assay Development Service


AnaSpec, provider of the long wavelength, ultra-sensitive SensoLyte assay kits, is pleased to offer custom service for the development of assays. Our long wavelength SensoLyte protease assays, many of which utilize the 5-FAM/QXL 520 FRET pair, have revolutionized the industry, setting the gold standard in fluorimetric assay sensitivity (Figure 1). Our expertise in peptide chemistry, combined with extensive experience in the syntheses of fluorescent dyes and quenchers, have resulted in the development of ultra-sensitive, convenient, time-saving, cost-effective assays. Researchers from the diagnostic, biotechnology, pharmaceutical and academic sectors have reported the use of our assays in numerous publications in high impact journals.

Committed to the highest levels of performance, our custom assay development team will work with you to develop assays that will meet or exceed your specific manufacturing and research priorities. Complete confidentiality of all orders is assured.

Design of optimized peptide substrates
FRET, fluorimetric or luminometric technology
Novel long wavelength FRET pairs, such as 5-FAM/QXL 520
Homogeneous assay, ideal for HTS (96 or 384-well format)
Assay validation with purified enzyme and/or known inhibitor
Assay parameters information included (sensitivity, linear range)
Detailed protocol provided

Figure 1. The 5-FAM/QXL 520 renin FRET substrate is 40X more sensitive than the traditional EDANS/DABCYL FRET substrate and 10X more sensitive than an Amp/Dnp FRET substrate. It can detect 0.8 ng/ml of renin.

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Select literature citations:

SensoLyte520 Renin Assay Kits

Du, D. et al. Mol. Biotech. 43, 1559 (2009).
Schmiedt, C. et al. Am. J. Vet. Res. 70, 1315 (2009).
Wei, Y. et al. Bioorganic and Medicinal Chem 17, 3003 (2009).
ivn, M. et al. Am. J. Hum. Genet. 85, 204 (2009).

SensoLyte 520 MMP Assay Kits

Spiga, MG. And T. Borras Invest. Ophthal. Vis. Sc. doi:10.1167/iovs.09-4918 (2010).
Corriveau, MP. et al. J. Pathol. 217, 534 (2009).
Gueders, MM. et al. Biochem. Pharma. 75, 514 (2008).
Adiseshaiah, P. et al. J. Cell. Physiol. 216, 405 (2008).
Johnson, SL. et al. Bioorganic Chem. 35, 306 (2007).
Yi, F. et al. Kidney Int. 70, 88 (2006).

SensoLyte 520 TACE Assay Kits

Hassemer, EL. et al. Genetics doi:10.1534/genetics.109.113167 (2010).
Hurst, LA. et al. J Neuroimmunol 210, 108 (2009).
Walker, EJ. and GA. Rosenberg Exp Neuro 216, 122(2009).
Cardellini, M. et al. Diabetes 58, 2396 (2009).