Mant-ATP & Mant-GTP - NEW


AnaSpec is pleased to announce the release of Mant-ATP and Mant-GTP.

Mant-ATP and Mant-GTP are the fluorescent analogs of ATP and GTP where either the ribose 2’-hydroxy or the 3’-hydroxy group is esterified by the fluorescent methylisatoic acid (Figure 1). The fluorescence quantum yield of Mant fluorophore is 0.2 in water, which increases significantly in nonpolar solvents or upon binding to most proteins. The highly environmentally sensitive fluorescence of Mant makes Mant-ATP and Mant-GTP useful probes for directly detecting nucleotide-protein interactions at Ex/Em=355/448 nm.

With >95% purity, AnaSpec’s Mant-ATP and Mant-GTP offer cost-effective value without compromising performance.


Figure 1. Structures of Mant-ATP (left) and Mant-GTP (right).

Product

Size

Catalog #

Mant-GTP [2'-/3'-O-(N'-Methylanthraniloyl)guanosine-5'-O-triphosphate, trisodium salt],5mM in TE buffer(pH7.6) NEW

500 µL

64609

Mant-ATP [2'-/3'-O-(N'-Methylanthraniloyl)adenosine-5'-O-triphosphate, trisodium salt],5mM in TE buffer(pH7.6) NEW

500 µL

64610

References:

1. Mocz, G. et al. Probing the Nucleotide Binding Sites of Axonemal Dynein with the Fluorescent Nucleotide Analogue 2'(3')-O-(-N-Methylanthraniloyl)-adenosine 5'-triphosphate. Biochemistry, 1998, 37, 9862-9869.

2. Bauer, C.B. et al. X-ray Crystal Structure and Solution Fluorescence Characterization of Mg-2'(3')-(N-Methylanthraniloyl) Nucleotides Bound to the Dictyostelium discoideum Myosin Motor Domain. J. Mol. Biol., 1997, 274, 394-407.

3. Hiratsuka, T., New Ribose-modified Fluorescent Analogs of Adenine and Guanine Nucleotides Available as Substrates for Various Enzymes. Biochim. Biophys. Acta, 1983, 742, 496-508.

4. Cunliffe, J.M. et al. Detection of Adenylyl Cyclase Activity Using a Fluorescent ATP Substrate and Capillary Electrophoresis. Anal. Chem., 2006, 78, 1731-1738.

5. Mou, T.-C. et al. Structural Basis for the Inhibition of Mammalian Membrane Adenylyl Cyclase by 2'(3')-O-(N-Methylanthraniloyl)-guanosine 5'-triphosphate. J. Biol. Chem., 2005, 280, 7253-7261.

6. Remmers, A. E. et al. Fluorescent Guanine Nucleotide Analogs and G Protein Activation. J. Biol. Chem., 1994, 269, 13771-13778

7. Hazlett, T.L. et al. Solution Dynamics of p21ras Proteins Bound with Fluorescent Nucleotides: a Time-resolved Fluorescence Study. Biochemistry, 1993, 32, 13575-13583.

8. Eccleston, J.F. et al. Fluorescence Approaches to the Study of the p21ras GTPase Mechanism. Biochem. Soc. Trans., 1991, 19, 432-437.