AnaSpec is pleased to announce the release of Mant-ATP and
Mant-GTP.
Mant-ATP and Mant-GTP are the fluorescent analogs of ATP and
GTP where either the ribose 2’-hydroxy or the 3’-hydroxy group is esterified
by the fluorescent methylisatoic acid (Figure 1). The fluorescence
quantum yield of Mant fluorophore is 0.2 in water, which increases significantly
in nonpolar solvents or upon binding to most proteins. The highly environmentally
sensitive fluorescence of Mant makes Mant-ATP and Mant-GTP useful probes for
directly detecting nucleotide-protein interactions at Ex/Em=355/448 nm.
With >95% purity, AnaSpec’s Mant-ATP and Mant-GTP offer
cost-effective value without compromising performance.
Figure 1. Structures of Mant-ATP (left) and Mant-GTP (right).
References:
1.
Mocz, G. et al. Probing the Nucleotide Binding Sites of Axonemal Dynein with
the Fluorescent Nucleotide Analogue 2'(3')-O-(-N-Methylanthraniloyl)-adenosine
5'-triphosphate. Biochemistry, 1998, 37, 9862-9869.
2.
Bauer, C.B. et al. X-ray Crystal Structure and Solution Fluorescence Characterization
of Mg-2'(3')-(N-Methylanthraniloyl) Nucleotides Bound to the Dictyostelium discoideum
Myosin Motor Domain. J. Mol. Biol., 1997, 274, 394-407.
3.
Hiratsuka, T., New Ribose-modified Fluorescent Analogs of Adenine and Guanine
Nucleotides Available as Substrates for Various Enzymes. Biochim. Biophys.
Acta, 1983, 742, 496-508.
4.
Cunliffe, J.M. et al. Detection of Adenylyl Cyclase Activity Using a Fluorescent
ATP Substrate and Capillary Electrophoresis. Anal. Chem., 2006, 78,
1731-1738.
5.
Mou, T.-C. et al. Structural Basis for the Inhibition of Mammalian Membrane
Adenylyl Cyclase by 2'(3')-O-(N-Methylanthraniloyl)-guanosine 5'-triphosphate.
J. Biol. Chem., 2005, 280, 7253-7261.
6.
Remmers, A. E. et al. Fluorescent Guanine Nucleotide Analogs and G Protein Activation.
J. Biol. Chem., 1994, 269, 13771-13778
7.
Hazlett, T.L. et al. Solution Dynamics of p21ras Proteins Bound with Fluorescent
Nucleotides: a Time-resolved Fluorescence Study. Biochemistry, 1993,
32, 13575-13583.
8.
Eccleston, J.F. et al. Fluorescence
Approaches to the Study of the p21ras GTPase Mechanism. Biochem. Soc. Trans.,
1991, 19, 432-437.
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