The hallmark
of Alzheimer’s disease (AD) pathology includes β-sheet aggregates of β-amyloid
peptides in senile plaques and hyperphosphorylated tau protein in neurofibrillary
tangles (NFT).1-2 Recent publications have reported the use of Cys-containing
mutants as models for aggregation studies.3-5 Shivaprasad and Wetzel
employ “the use of disulfide bond cross-linking to probe the fold within the
core and the packing interactions between beta-sheets.” Among the
Cys mutants they studied is the S26C (Ser substituted with Cys at position 26).3
Upon oxidation of the S26C monomer, cysteines are capable of forming intermolecular
disulfide bond creating the S26C dimer.3-4 Using this synthetic dimer,
Hu, et al. show that it behaves similarly to β-amyloid dimer-containing
human CSF, suggesting that Aβ dimers may be the earliest synaptic
disrupting species in AD.4
As the supplier of the world’s largest collection of β-amyloid
GO™ (catalog) Peptides, AnaSpec is pleased to add to this list,β-amyloid (1-40) S26C;
β-amyloid (1-40) S26C dimer;
β-amyloid (1-42) S26C and
other Cys-containing β-amyloid peptides (Cys on the N or C-terminus as
well as in the internal sequence).
Besides using these Cys-containing β-amyloid peptides for dimerization,
the availability of the Cysteine’s thiol group also allows researchers the flexibility
of reacting these peptides with any maleimide containing fluorescent dyes or
biomolecules. For example, the thiol group of Cys-β-amyloid (1-40) can
react with HiLyte
Fluor™ 488, C2 maleimide to form HiLyte Fluor™ 488-Cys-β-amyloid (1-40)
[Cys(HiLyte Fluor™ 488)-DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV].
Click here
for more information.
Table 1. Cys-containing
β-amyloid (1-40) and β-amyloid (1-42).
| Product |
Size |
Catalog # |
| Cys - beta - Amyloid (1 - 40)
CDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV |
0.5 mg |
23519
23520 |
| [Cys7] - beta - Amyloid (1 - 40)
DAEFRHCSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV |
0.5 mg
1 mg |
62815-05
62815-1 |
| [Cys20] - beta - Amyloid (1 - 40) NEW
DAEFRHDSGYEVHHQKLVFCAEDVGSNKGAIIGLMVGGVV |
0.5 mg |
63895 |
| [Cys26] - beta - Amyloid (1 - 40),
S26C beta - Amyloid (1 - 40)
DAEFRHDSGYEVHHQKLVFFAEDVGCNKGAIIGLMVGGVV |
0.5 mg
1 mg |
63671-05
63671-1 |
| [Cys26] - beta - amyloid (17 - 40),
S26C beta - amyloid (17 - 40) NEW
LVFFAEDVGCNKGAIIGLMVGGVV |
1 mg |
63866 |
| Beta-Amyloid (1-40) (S26C) Dimer NEW
DAEFRHDSGYEVHHQKLVFFAEDVGCNKGAIIGLMVGGVV (dimer) |
0.5 mg
1 mg |
64130-05
64130-1 |
| Beta - Amyloid (1 - 40) - Cys
DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVC |
0.1 mg |
62233 |
| Cys - containing beta - Amyloid (1 - 40) Binding
Peptide, Biotin - labeled
Biotin
- AECDWGKGGRWRLWPGASGKTEACGP |
1 mg |
62429 |
| [Cys26] - beta - Amyloid (1 - 42),
S26C beta - Amyloid (1 - 42)
DAEFRHDSGYEVHHQKLVFFAEDVGCNKGAIIGLMVGGVVIA |
0.5 mg
1 mg |
63672-05
63672-1 |
| Cys - beta - Amyloid (1 - 42)
CDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA |
|
23537
23538 |
Table 2. Additional
N-terminal Cys-containing β-amyloid peptides
| Product |
Size |
Catalog # |
| Cys - beta - Amyloid (1 - 11)
CDAEFRHDSGYE |
1 mg |
64351 |
| Cys - beta - Amyloid (1 - 12)
CDAEFRHDSGYEV |
1 mg |
64353 |
| Cys - beta - Amyloid (4 - 10)
CFRHDSGY |
1 mg |
61979 |
| Cys - beta - Amyloid (12 - 28)
CVHHQKLVFFAEDVGSNK |
0.1
mg
0.5 mg
1 mg |
23548-01
23547
23548 |
| Cys - beta - Amyloid (25 - 35) NEW
CGSNKGAIIGLM |
1 mg |
63869 |
| Cys - Amyloid Precursor Protein (APP) (751
- 770)
CKMQQNGYENPTYKFFEQMQN |
1 mg |
63302 |
| [Cys40] - beta - hairpin (BHA),
Protein G B1 Domain (41 - 56)
CGEWTYDDATKTFTVTE |
1 mg |
62533 |
| Cys - NC2 - 2 CLAC - P (155 - 169)
CKGEQGDQGPRMVFPK |
1 mg |
62905 |
Table 3. C-terminal
Cys containing β-amyloid peptides
| Product |
Size |
Catalog # |
| Beta - Amyloid (1 - 5) - Cys
DAEFRC |
1 mg |
64352 |
| Beta - Amyloid (1 - 8) - Cys
DAEFRHDSC |
1 mg |
62132 |
| Beta - Amyloid (1 - 9) – Gly – Gly – Cys
DAEFRHDSGGGC |
1 mg |
64350 |
| Beta - Amyloid (1 - 10) – Cys
DAEFRHDSGYC |
1 mg |
62144 |
| Beta - Amyloid (1 - 12) - Cys - NH2
DAEFRHDSGYEVC - NH2 |
1 mg |
63333 |
| Beta - Amyloid (1 - 16) - Cys
DAEFRHDSGYEVHHQKC |
1 mg |
63746 |
| Beta - Amyloid (1 - 17) - Cys
DAEFRHDSGYEVHHQKLC |
1 mg |
62473 |
| Beta - Amyloid (1 - 24) - Cys
DAEFRHDSGYEVHHQKLVFFAEDVC |
1 mg |
62475 |
| Beta - Amyloid (4 - 24) - Cys
FRHDSGYEVHHQKLVFFAEDVC |
1 mg |
63326 |
| Beta - Amyloid (12 - 28) - Cys
VHHQKLVFFAEDVGSNKC |
0.1
mg
0.5 mg
1 mg |
23550-01
23549
23550 |
| Beta - Amyloid (13 - 29) - Gly - Cys
HHQKLVFFAEDVGSNKGGC |
1 mg |
61800 |
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References:
1. Khachaturian,
ZS. Arch. Neurol. 42, 1097 (1985).
2. Mirra, SS. et al. Neurol. 41, 479 (1991).
3. Shivaprasad, S.
and R. Wetzel. J. Biol. Chem. 281, 993 (2006).
4. Hu, N-W.
et al. Brain doi:10.1093/brain/awn174.
5. Shankar, GM. et
al. Nat. Med. 14, 837 (2008).
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