AnaSpec, Eurogentec Group, is pleased to be the first in the industry
to establish a new product line of zebrafish specific (Z-Fish™) antibodies
to support the increasing needs of researchers.
Our expanding list of Z-Fish™ antibodies includes targets used
in transcriptional regulation, signal transduction, apoptosis and autophagy,
cell cycle control, cytoskeleton, glucose and energy metabolism, and immunity
research. Antibodies were verified in ELISA. Many were verified in western blots
using zebrafish lysates.
As well, these Z-Fish™ antibodies are predicted to work in IHC, many
of which have good IHC results. Antibodies are supplied in 150 ul size, this
amount is enough for 15 mini gels.
In collaboration with the University
of Utah, Salt Lake City, Utah, AnaSpec's Z-Fish™ polyclonal anti-lysozyme was
found to completely overlap with mpo:GFP labeled neutrophils. This indicates
that lysozyme is a neutrophil marker in zebrafish (Figure 1). Lysozyme C (lyz), a cationic
glycoside hydrolase, breaks down N-acetyl muramic b-1,4 N-acetyl glucosamine linkages in the bacterial cell
wall.1-2 In zebrafish, this enzyme is expressed exclusively in myeloid
cells,2 and is used for hematopoietic developmental studies in zebrafish.
This antibody was raised using a synthetic peptide derived from
the intermediate region of zebrafish lysozyme protein (GenBank accession # NP_631919.1).
| Product |
Size |
Catalog
# |
| Anti-Lysozyme (IN),
Z-Fish™
Host: rabbit polyclonal; Species reactivity: zebrafish;
Application: IHC |
150 uL |
55633 |
Figure 1. Whole-mount zebrafish IHC of a 3 dpf larvae
probed with Z-Fish™ anti-lysozyme (IN), (cat# 55633) at 1:25-1:200 dilution.
Fixation conditions were 4% PFA with 0.1% Tween 20 overnight at 4 degrees. Anti-Lysozyme
antibody stains neutrophils in red. Anti-Lysozyme label
overlaps almost completely with the mpo:GFP labeled neutrophils, indicating that
lysozyme is a neutrophil marker (image courtesy of Dr. Michael Redd, Univ. of Utah).
References:
- Gordon, S. et al. J Exp Med 139(5), 1228 (1974).
- Kitaguchi, T. et al. Mech Dev 126, 314 (2009).
Related Products:
Zebrafish Lysates
Antibodies
for the following targets:
Antigen
Presentation
Apoptosis
and Autophagy
Binding
and Transportation
Cancer
Cell Cycle
Control
Cell Markers
Cell-Cell
and Cell Matrix Adhesion
Chemokine
Cytokine
Cytoskeleton
Enzyme
/ Co-Enzyme
Extracellular
Matrix/ MMP/ TIMP
Growth
Factor
Hematology
Hormone
/ Growth Factor
Immunoglobulin
Neuroscience
Oxidation
Protease
Protein
Binding and Protein Folding
Receptor
/ Co-Receptor
Signal
Transduction and Others
Transcription
Factors
Transcriptional
Regulation
|
