MMP Inhibitors


Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. 1,2 They are involved in many aspects of both physiological processes and pathological conditions such as tumor development and metastasis, 3,4 rheumatoid arthritis. 5 As a result, compounds that inhibit MMP activity are considered promising therapeutic agents.6

AnaSpec, Eurogentec Group, is pleased to release 11 MMP inhibitors to complement our wide offering of MMP SensoLyte® 520 and 490 fluorimetric kits, colorimetric kits, recombinant proteins, and Z-Fish™ antibodies (antibodies specific for zebrafish). These MMP inhibitors are small molecules and act as either potent broad-spectrum inhibitors or selective inhibitors of MMPs. They can be used as reference compounds for validating new MMP assays, and/or for identifying novel MMP inhibitors.

Table 1. A listing of MMP Inhibitors

Product Size Catalog #

ARP 101
CAS# 849773-64-4

5 mg

72190

ARP100
CAS# 704888 - 90 - 4

5 mg

72181

Batimastat
CAS#
130370-60-4

1 mg

72191

CL - 82198 hydrochloride
CAS# 307002-71-7

10 mg

72192

CP - 471474
CAS# 210755 – 45 - 6

10 mg

72195

Marimastat
CAS# 154039 - 60 - 8

1 mg

72183

ONO - 4817
CAS#223472 – 31 - 9

10 mg

72193

PD166793
CAS# 199850 - 67 - 4

10 mg

72180

Ro 32 - 3555
CAS#190648-49-8

10 mg

72194

UK370106
CAS# 230961 - 21 - 4

10 mg

72182

WAY170523
CAS# 307002 - 73 - 9

1 mg

72184


Related Products

SensoLyte® 520 MMP Assays

• Measure MMP activity
• 96-well or 384-well format
• Mix and read
• Fluorimetric - long wavelength
• 5-FAM/QXL™ 520 FRET pair (Ex/Em=490/520 nm)
• Minimal autofluorescence
• Assay time – as short as 1h

Figure 1. The performance of an identical peptide sequence, one coupled with 5-FAM/QXLTM 520 and the other coupled with EDANS/DABCYL FRET pairs was compared (A). Fluorescence was measured after 1 hr incubation of FRET substrates with serial dilutions of MMP-13 (Flex800, Bio-Tek instruments). A 5-FAM/QXLTM 520 MMP substrate was compared with Mca/Dpa FRET substrate (B). Fluorescence was measured after 1 hr incubation of substrates using serial dilutions of MMP-13 (Flexstation 384II, Molecular Devices).

SensoLyte® Plus 520 MMP Assays

• Measure MMP activity
• Enhanced specificity – human capture antibody included
• 96-well format (strips)
• Mix and read
• Fluorimetric - long wavelength
• 5-FAM/QXL™ 520 FRET pair (Ex/Em=490/520 nm)
• Minimal autofluorescence

SensoLyte® 520 Series

SensoLyte Plus® 520 Series

SensoLyte® 490 Series

SensoLyte® ELISA Series

Recombinant Human MMPs

MMP-1

71150

72012

71128

72102

72004

MMP-2

71151

71129

72005

MMP-3

71152

71130

72103

72006

MMP-7

71153

71132

72007

MMP-8

71154

71133

72104

72008

MMP-9

71155

72017

71134

72105

72009

72069 (mouse)

MMP-10

72024

72106

72067

MMP-12

71157

71137

72010

MMP-13

71156

72019

71135

72107

72011

MMP-14

72025

72068

SensoLyte® 490 MMP Assays

• Measure MMP activity
• 96-well or 384-well format
• Mix and read
• Fluorimetric
• EDANS/DABCYL FRET pair (Ex/Em=340/490 nm), except 71132 NBD/DMC FRET pair (Ex/Em=370/460 nm)
• Assay time – as short as 1h

Also available:
SensoLyte® 520 Generic MMP Assay Kit
*Fluorimetric*
SensoLyte® 570 Generic MMP Assay Kit *Fluorimetric*
SensoLyte® Generic MMP Assay Kit “Colorimetric”
SensoLyte® 520 MMP Substrate Sampler Assay Kit *Fluorimetric*
SensoLyte® 520 MMP Profiling Assay Kit *Fluorimetric*

SensoLyte® MMP ELISA Assays

• Measure MMP amount
• 96-well format
• Colorimetric (Absorbance)

MMP Recombinant Proteins

• 100 ul (10 ug/ml)
• Highly active after APMA incubation
• Serve as positive controls for SensoLyte® MMP assay kits
• Can also be used for
zymography, Western blot, IP, and ELISA

Anti-MMP Antibodies

• Human, zebrafish (Z-Fish™), mouse and rat specific
• Epitopes from hinge, C-terminal (CT), N-terminal (NT), IN (internal) regions

References

1. Woessner, JF. Jr. et al. J. Biol. Chem. 263, 16918 (1988).
2.
Woessner, JF. Jr. FASEB J. 5, 2145 (1991).
3.
Goldberg, GI. et al. Ann. N.Y. Acad. Sci. 580, 375 (1990).
4.
Stetler-Stevenson, WG. et al. Ann. Rev. Cell Biol. 9, 541 (1993).
5.
Gravallese, EM. et al. Arthritis Rheum. 34, 1076 (1991).
6.
Levy, DE. et al. J Med Chem 41, 199 (1998).