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BLUE qPCR Mastermixes

For both FAST and regular protocols, Eurogentec's MESA BLUE qPCR MasterMix Plus for SYBR^® Assay is a unique combination of our high-performance Hotstart Meteor Taq and an inert BLUE dye. The BLUE dye, which does not affect the qPCR reaction considerably enhances the contrast between the reagent and plasticware, making verification of MasterMix dispensing quick, easy and foolproof, especially when using white plates.

MESA BLUE qPCR MasterMixes, tailored for every thermocycler requirement, are ready-to-use, easy-to-see formulated mixes. These kits provide consistent and sensitive results across all different qPCR platforms and prevent aberrant results before they occur.

• For SYBR assay with both FAST and REGULAR protocols
• Visualize dispensing errors at a glance
• Optimized for maximal sensitivity
• Maximum consistency and specificity
• Easy-to-see & easy-to-use
• Increased signal on ABI platforms

Ct MESA BLUE	12.69	15.74	19.5	22.96	26.88	30.5
Ct Competitor A	13.56	17.05	20.55	24.27	27.64	31.35

Figure 1. Comparison of the MESA BLUE MasterMix Plus for SYBR Assay and competitor A shows increased sensitivity, steeper amplification curves of the MESA BLUE MasterMix Plus (No ROX). Experiments were performed using the same cDNA and18S primers on a MX3000P^R.

Table 1. A MESA BLUE mastermix for every thermocycler requirement.

Product*	Size	Catalog #
MESA BLUE qPCR MasterMix Plus for SYBR^R Assay	600 RXNs (7.5 ml)	RT-SY2X-03+WOUB
MESA BLUE qPCR MasterMix Plus for SYBR^R Assay, Low ROX	600 RXNs (7.5 ml)	RT-SY2X-03_WOULRB
MESA BLUE qPCR MasterMix Plus for SYBR^R Assay, No ROX	600 RXNs (7.5 ml)	RT-SY2X-03+NRWOUB
MESA BLUE qPCR MasterMix Plus for SYBR^R Assay, w/fluorescein	600 RXNs (7.5 ml)	RT-SY2X-03+WOUFLB

* Use ROX mixes on ABI (except 7500). Use Low ROX mixes on ABI 7500 and Stratagene (when ROX normalization is required) machines. Use No ROX mixes on all other machines.