BLUE qPCR Mastermixes


For both FAST and regular protocols, Eurogentec's MESA BLUE qPCR MasterMix Plus for SYBR Assay is a unique combination of our high-performance Hotstart Meteor Taq and an inert BLUE dye. The BLUE dye, which does not affect the qPCR reaction considerably enhances the contrast between the reagent and plasticware, making verification of MasterMix dispensing quick, easy and foolproof, especially when using white plates.

MESA BLUE qPCR MasterMixes, tailored for every thermocycler requirement, are ready-to-use, easy-to-see formulated mixes. These kits provide consistent and sensitive results across all different qPCR platforms and prevent aberrant results before they occur.

For SYBR assay with both FAST and REGULAR protocols
Visualize dispensing errors at a glance
Optimized for maximal sensitivity
Maximum consistency and specificity
Easy-to-see & easy-to-use
Increased signal on ABI platforms

Ct MESA BLUE

12.69

15.74

19.5

22.96

26.88

30.5

Ct Competitor A

13.56

17.05

20.55

24.27

27.64

31.35


Figure 1. Comparison of the MESA BLUE MasterMix Plus for SYBR Assay and competitor A shows increased sensitivity, steeper amplification curves of the MESA BLUE MasterMix Plus (No ROX). Experiments were performed using the same cDNA and18S primers on a MX3000PR.


Table 1. A MESA BLUE mastermix for every thermocycler requirement.

Product*

Size

Catalog #

MESA BLUE qPCR MasterMix Plus for SYBRR Assay

600 RXNs (7.5 ml)

RT-SY2X-03+WOUB

MESA BLUE qPCR MasterMix Plus for SYBRR Assay, Low ROX

600 RXNs (7.5 ml)

RT-SY2X-03_WOULRB

MESA BLUE qPCR MasterMix Plus for SYBRR Assay, No ROX

600 RXNs (7.5 ml)

RT-SY2X-03+NRWOUB

MESA BLUE qPCR MasterMix Plus for SYBRR Assay, w/fluorescein

600 RXNs (7.5 ml)

RT-SY2X-03+WOUFLB

* Use ROX mixes on ABI (except 7500). Use Low ROX mixes on ABI 7500 and Stratagene (when ROX normalization is required) machines. Use No ROX mixes on all other machines.

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qPCR Mastermixes for SYBRR
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Universal Reverse Transcriptase Kits