The introduction of tags, whether short peptides (6X-His, DYKDDDDK,
c-myc) or proteins (green fluorescent protein – GFP, glutathione-s-transferase
– GST) into recombinant proteins aids in the characterization, localization
or purification of the recombinant expressed protein.1-3 As a consequence,
antibodies raised against fusion tags are useful in biochemical or immunohistochemistry
applications.
AnaSpec, EGT Group is pleased to offer a wide selection of high
quality monoclonal and polyclonal antibodies against the hexa-His and GFP
tags.
• Wide
selection of anti-His and anti-GFP antibodies
• Polyclonal
and monoclonal antibodies
• Fluorophore
or biotin-labeled His tag antibodies available
Table 1. Anti-His tag antibodies are available as polyclonal or monoclonal
antibodies
Table 2. A listing of Anti-GFP antibodies.
| Product |
Size |
Catalog
# |
| Anti-GFP (1A5), monoclonal |
100 ug |
55887 |
| Anti-GFP Tag (6A2B3), monoclonal |
100 ug |
55886 |
| Anti-GFP Tag |
100 ug
500 ug
1 mg |
29779
29779-500
29779-1000 |
|
Anti-GFP Tag (CT )
|
100 ug |
53882 |
| Anti-GFP Tag (NT)
|
100 ug |
54045 |
| Anti-GFP Tag (IN)
|
100 ug |
53214 |
| Anti-GFP-Tag, Chicken IgY
|
100 ug |
55423 |
Figure 1. ICC of HEK293 cells transfected with GFP and probed
with polyclonal Anti-GFP (Cat# 29779). Secondary antibody used was Goat anti-rabbit,
Hilyte Fluor™ 555 labeled (red). Nucleus counter-stained with DAPI.
Fluorescently labeled and biotin labeled 6X-His antibodies are also available.
Antibodies are labeled with a selection of dyes, including our proprietary HiLyte
Fluor™ dyes, which span the visible spectrum. This gives researchers
a wide range of wavelength choices for use in immunohistochemistry or immunofluorescence
applications.
Table 3. A listing of biotin and dye-labeled polyclonal Anti-His antibodies.
| Product |
Size |
Catalog
# |
| Polyclonal Anti-His Tag IgG, Biotinylated |
100 µg |
29673-BIOT |
| Polyclonal Anti-His Tag IgG, FITC labeled
(Ex/Em=495/520 nm) |
100 µg |
29673-FITC |
| Polyclonal Anti-His Tag IgG, HiLyte Fluor™
405-labeled (Ex/Em=404/428 nm) |
100 µg |
29673-H405 |
| Polyclonal Anti-His Tag IgG, HiLyte Fluor™
488 labeled (Ex/Em=499/523 nm) |
100 µg |
29673-H488 |
| Polyclonal Anti-His Tag IgG, HiLyte Fluor™
555 labeled (Ex/Em=553/568 nm) |
100 µg |
29673-H555 |
| Polyclonal Anti-His Tag IgG, HiLyte Fluor™
594 labeled (Ex/Em=593/616 nm) |
100 µg |
29673-H594 |
| Polyclonal Anti-His Tag IgG, HiLyte Fluor™
647 labeled (Ex/Em=653/673 nm) |
100 µg |
29673-H647 |
Table 4. A listing of biotin and dye-labeled monoclonal Anti-His antibodies.
| Product |
Size |
Catalog
# |
| Monoclonal Anti-His Tag IgG, Biotinylated |
100 µg |
61250-BIOT |
| Monoclonal Anti-His Tag IgG, AMCA labeled
(Ex/Em=344/446 nm) |
100 µg |
61250-AMCA |
| Monoclonal Anti-His Tag IgG, FAM labeled
(Ex/Em=495/520nm) |
100 µg |
61250-FAM |
| Monoclonal Anti-His Tag IgG, FITC labeled
(Ex/Em=495/520nm) |
100 µg |
61250-FITC |
| Monoclonal Anti-His Tag IgG, ROX labeled
(Ex/Em=573/602 nm) |
100 µg |
61250-ROX |
| Monoclonal Anti-His Tag IgG, TAMRA labeled
(Ex/Em=555/580 nm) |
100 µg |
61250-TAMR |
| Monoclonal Anti-His Tag IgG, HiLyte Fluor™
405-labeled (Ex/Em=404/428 nm) |
100 µg |
61250-H405 |
| Monoclonal Anti-His Tag IgG, HiLyte Fluor™
488 labeled (Ex/Em=499/523 nm) |
100 µg |
61250-H488 |
| Monoclonal Anti-His Tag IgG, HiLyte Fluor™
555 labeled (Ex/Em=553/568 nm) |
100 µg |
61250-H555 |
| Monoclonal anti-His Tag IgG, HiLyte Fluor™
594-labeled (Ex/Em=593/616 nm) |
100 µg |
61250-H594 |
| Monoclonal Anti-His Tag IgG, HiLyte Fluor™
647 labeled (Ex/Em=653/673 nm) |
100 µg |
61250-H647 |
Related Products
Full listing
of tag antibodies
Speedy
28-Day Polyclonal Antibody Service
70-Day
Custom Polyclonal Antibody Service
Custom
Hybridoma Development and Monoclonal Antibody Production
References:
1. Zheng et al. J Biochem 133, 577 (2003).
2. Amrein et al. PNAS 92, 1048 (1995).
3. Nakajima K. and Yaoita Y. Nucleic Acids Res 25, 2231 (1997).
4. Medina et al. Nucleic Acids Res 28, I (2000).
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