SensoLyte 520 Renin Assays human, mouse & rat specific


AnaSpec, EGT Group, the provider of the ultra-sensitive SensoLyte 520 Human Renin Assay Kit also provides renin kits specific for mouse and rat - the SensoLyte 520 Mouse Renin Assay Kit and SensoLyte 520 Rat Renin Assay Kit. Using our proprietary 5-FAM/QXL 520 fluorescence resonance energy transfer (FRET) peptide, all three assays are ideal for screening of renin inhibitors and for continuous assay of renin activity, especially for high throughput screening (HTS) purposes. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL 520. However, upon cleavage into two separate fragments by rat renin, the fluorescence of 5-FAM is recovered, and can be monitored at excitation/emission = 490/520 nm. With a high fluorescence quantum yield and long emission wavelength, the signal of 5-FAM can be detected with less interference from the autofluorescence of cell components and test compounds. The assays are performed in a convenient 96-well microplate format and can be adjusted to 384-well microplate format.

Ultra-sensitive assays
FRET based substrates
Long wavelength
Minimal autofluorescence
Renin assays kits specific for human, mouse & rat

The reninangiotensin system (RAS) plays a central role in the regulation of blood pressure and electrolyte homoeostasis.1 At the first and rate-limiting step of the RAS cascade, renin, a highly specific aspartyl protease, cleaves angiotensinogen, produced in the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE (Angiotensin Converting Enzyme). Angiotensin II constricts blood vessels leading to increased blood pressure. It also increases the secretion of ADH and aldosterone, and stimulates the hypothalamus to activate the thirst reflex. Since an overactive renin-angiotensin system leads to hypertension, renin is an attractive target for the treatment of this disease. 2-4

SensoLyte 520 Renin Assay Kit (cat# 72040)

SensoLyte 520 Mouse Renin Assay Kit (cat# 72161)

SensoLyte 520 Rat Renin Assay Kit (cat# 72140)

FRET pair

5-FAM/QXL 520

5-FAM/QXL 520

5-FAM/QXL 520

Ex/Em (nm)

490/520

490/520

490/520

Min. Detectable Renin

0.8 ng/ml (using human recombinant renin)

30 ng/ml (using mouse recombinant renin)

0.9 ug/ml (using rat recombinant renin)

Interference from Autofluorescence

Minimal

Minimal

Minimal












Figure 1. Comparison data of 3 renin assay kits. SensoLyte 520 Human Renin Assay Kit (Cat# 72040), SensoLyte 390 Renin Assay Kit (Cat# 72039) and a kit from company B.



Figure 2. A schematic representation of the cleavage of a FRET peptide substrate by renin is shown on top. The absorption spectrum of QXL 520 perfectly overlaps with the emission spectrum of 5-FAM (bottom).

Recent Citations

SensoLyte 520 Human Renin Assay Kit

Schmiedt, CW. et al. (2012). Bilateral renal ischemia as a model of acute kidney injury in cats. Res Vet Sc doi.org/10.1016/j.rvsc.2011.12.004.
Yoshikawa, A. et al. (2011). The (pro)renin receptor is cleaved by ADAM19 in the Golgi leading to its secretion into extracellular space. Hpertension 34, 599.
May, PC. et al. (2011). Robust central reduction of amyloid-β in humans with an orally available, non-peptidic β-secretase inhibitor. J Neurosc 31, 16507. doi: 10.1523/JNEUROSCI.3647-11.2011
Visavadiya, NP. et al. (2011). High glucose upregulates upstream stimulatory factor 2 in human renal proximal tubular cells through angiotensin II-dependent activation of CREB. Exp Nephro doi: 10.1159/000320593. Sbroggio, M. et al. (2011). IQGAP1 regulates ERK1/2 and AKT signaling in the heart and sustains functional remodeling upon pressure overload. Cardiovasc Res doi: 10.1093/cvr/cvr103.

SensoLyte 520 Rat Renin Assay Kit

Chelko, SP. et al. (2012). A novel vascular clip design for the reliable induction of 2-kidney, 1-clip hypertension in the rat. J Appl Physio 112, 362.
Cheng, W-H. et al. (2012). Renin activates PI3K-Akt-eNOS signaling through the AT1 receptor and Mas receptor to modulate central blood pressure control in the nucleus tractus solitarii. Brit J Pharma DOI: 10.1111/j.1476-5381.2012.01832.x.

SensoLyte 520 Mouse Renin Assay Kit

Prysyazhna, O. et al. (2012). Single atom substitution in mouse protein kinase G eliminates oxidant sensing to cause hypertension. Nature Med 18, 286.

Related Products

Human Renin, Recombinant (5ug)
Mouse Prorenin, Recombinant (20 ug)
Rat Renin, Recombinant (20 ug)
SensoLyte 390 Renin Assay Kit
SensoLyte 390 ACE2 Activity Assay Kit
Angiotensins and Related Peptides
Natriuretic Peptides (ANP, BNP & CNP)

References:

1. He, FJ. and GA. MacGregor, J. Renin Angiotensin Aldosterone Syst. 4, 11 (2003).
2. Wood, JM. et al., Hypertension, 7, 797 (1985).
3. Shibasaki, M. et al., Am. J. Hypertens. 4, 932 (1991).
4. Wood, JM.et al., Biochem. Biophys. Res. Comm. 308, 698 (2003).