Labeling-detection

5(6)-CFDA, SE [5(6)-Carboxyfluorescein diacetate, SE] Fluorescent pH Indicator - 25 mg

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  • Cat.Number : AS-89000
  • Availability :
    In stock

Quantity

Reactive pH indicator for slightly acidic pH range

Specifications

Chemistry
CAS registry number
  • 150347-59-4
Molecular Formula
  • C29H19NO11
Molecular Mass/ Weight
  • 557.5
Properties
Absorbance (nm)
  • 492
Emission (nm)
  • 517
Color
Quantity & Purity
Purity
  • Peak Area by HPLC ≥90%
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • DMSO
Storage Conditions
  • -20°C Protected from Light
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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References

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  • M. Weinlich
  • et al

A long lifetime chemical sensor: study on fluorescence property of fluorescein isothiocyanate and preparation of pH chemical sensor

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  • LY Ma
  • et al

A rapid method for measuring intracellular pH using BCECF-AM

Biochim Biophys Acta . 2002 Aug 15 ; 1572(1) 143 | DOI : https://doi.org/10.1016/S0304-4165(02)00303-3

  • P. Ozkan
  • et al

Dual Excitation Ratiometric Fluorescent pH Sensor for Noninvasive Bioprocess Monitoring: Development and Application

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  • HR. Kermis
  • et al

Inadequacy of high K+/nigericin for calibrating BCECF. I. Estimating steady-state intracellular pH

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  • G. Boyarsky
  • et al

Inadequacy of high K+/nigericin for calibrating BCECF. II. Intracellular pH dependence of the correction

Am J Physiol . 1996 Oct 01 ; 271(4) 1146 | DOI : https://doi.org/10.1152/ajpcell.1996.271.4.C1146

  • G. Boyarsky
  • et al

Temperature and pH dependence of fluorescein binding within the monoclonal antibody 9-40 active site as monitored by hydrostatic pressure

J Biol Chem . 1996 Mar 01 ; 271-10 5332 | DOI : https://doi.org/10.1074/jbc.271.10.5332

  • J. Carrero
  • EW. Voss

Quenching of fluorescence of 1-hydroxypyrene-3,6,8-trisulfonate (HPTS) by Cu2+, Co2+, Ni2+, I−, and cetylpyridinium (CP+) ions in water/AOT/heptane microemulsion

J Colloid Interface Sci . 2004 Mar 03 ; 271(1) 157 | DOI : https://doi.org/10.1016/j.jcis.2003.09.038

  • S. Biswas

Rapid method for the preparation of a robust optical pH sensor

Analyst . 2003 Aug 19 ; 128 1181 | DOI : https://doi.org/10.1039/B304902G

  • HR. Kermis
  • et al