AnaSpec Europium (Eu) is an excellent tool for generating Eu-protein conjugates, and offers advantages over conventional fluorophores. Europium’s long-lived fluorescence and large Stoke’s shift allow for no background interference during assay measurements from the sample’s auto-fluorescence. The signal of Europium can be monitored at excitation/emission wavelengths = 325 nm/620 nm. Europium protein conjugates can be used in assay development for TR-FRET (time resolved-fluorescence resonance energy transfer) with red dyes as an acceptor such as HiLyte™ Fluor 647, either Cy5® or CyLyte, Alexa Fluor® 647 or APC. The kit has all the essential components for performing 1 conjugation reaction labeling up to 200 µg IgG and for purifying the Eu-protein conjugate. Kit Size: 1 labeling reaction (1 x 200 µg protein).
Note: AnaTag kits are not intended for peptide labeling.