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Labeling-detection

Biotin-X NTA [Biotin-X nitrilotriacetic acid, tripotassium salt] - 5 mg

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  • Cat.Number : AS-60655
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Biotin-X nitrilotriacetic acid, tripotassium salt (biotin-X NTA) is an excellent reagent for detecting polyhistidine-containing biomolecules immobilized on membranes.
The NTA moiety chelates a Ni++ ion that is also chelated with His tags. The complex can than be detected using streptavidin-conjugates such as streptavidin-HRP. Biotin-X NTA can be removed from the His-tagged molecule at pH 4.8, allowing the blot to be reprobed.

Specifications

Chemistry
CAS registry number
  • 856661-92-2
Molecular Formula
  • C26H40N5O9S.3K
Molecular Mass/ Weight
  • 716
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • Water
Storage Conditions
  • -20°C Protected from Light
Activity
Application
Biomarker Target
Research Area
Sub-category Research Area
Usage
  • Research use

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References

Detection and Quantitation of Hexa-Histidine-Tagged Recombinant Proteins on Western Blots and by a Surface Plasmon Resonance Biosensor Technique

Anal Biochem . 1995 Jul 01 ; 229(1) 119 | DOI : https://doi.org/10.1006/abio.1995.1387

  • DJ. Oshannessy
  • et al

Single-Step Synthesis and Characterization of Biotinylated Nitrilotriacetic Acid, a Unique Reagent for the Detection of Histidine-Tagged Proteins Immobilized on Nitrocellulose

Anal Biochem . 1996 Apr 05 ; 236(1) 101 | DOI : https://doi.org/10.1006/abio.1996.0137

  • SA. McMahan
  • R. Burgess