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Labeling-detection

Fluorescein Fluorescence Reference Standard - 1 g

$82.00
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  • Cat.Number : AS-80001
  • Availability :
    In production

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pH-dependent fluorescence; Maximum intensity observed in basic solutions.

Specifications

Chemistry
CAS registry number
  • 05/07/2321 00:00:00
Molecular Formula
  • C20H12O5
Molecular Mass/ Weight
  • 332.3
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 514
Color
Quantity & Purity
Purity
  • Peak Area by HPLC ≥95%
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • DMSO
Storage Conditions
  • -20°C Protected from Light
Activity
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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References

A long lifetime chemical sensor: study on fluorescence property of fluorescein isothiocyanate and preparation of pH chemical sensor

Spectrochim Acta A Mol Biomol Spectrosc . 2004 Jul 01 ; 60(8-9) 1865 | DOI : https://doi.org/10.1016/j.saa.2003.10.004

  • LY Ma
  • et al

A rapid method for measuring intracellular pH using BCECF-AM

Biochim Biophys Acta . 2002 Aug 15 ; 1572(1) 143 | DOI : https://doi.org/10.1016/S0304-4165(02)00303-3

  • P. Ozkan
  • et al

Dual Excitation Ratiometric Fluorescent pH Sensor for Noninvasive Bioprocess Monitoring: Development and Application

Biotechnol Prog . 2002 Sep 05 ; 18(5) 1047 | DOI : https://doi.org/10.1021/bp0255560

  • HR. Kermis
  • et al

In Vivo Application of Intestinal pH Measurement Using 2,7‘-Bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) Fluorescence Imaging

Photochem Photobiol . 1999 Nov 01 ; 70(5) 813 | DOI : https://doi.org/10.1111/j.1751-1097.1999.tb08287.x

  • X. Marechal
  • et al

Inadequacy of high K+/nigericin for calibrating BCECF. I. Estimating steady-state intracellular pH

Am J Physiol . 1996 Oct 01 ; 271(4) C1131 | DOI : https://doi.org/10.1152/ajpcell.1996.271.4.C1131

  • G. Boyarsky
  • et al

Simultaneous detection of cell volume and intracellular pH in isolated rat duodenal cells by confocal microscopy and BCECF

Res Exp Med (Berl) . 1998 Apr 04 ; 198 73 | DOI : https://doi.org/10.1007/s004330050091

  • M. Weinlich
  • et al

Quenching of fluorescence of 1-hydroxypyrene-3,6,8-trisulfonate (HPTS) by Cu2+, Co2+, Ni2+, I−, and cetylpyridinium (CP+) ions in water/AOT/heptane microemulsion

J Colloid Interface Sci . 2004 Mar 03 ; 271(1) 157 | DOI : https://doi.org/10.1016/j.jcis.2003.09.038

  • S. Biswas

Rapid method for the preparation of a robust optical pH sensor

Analyst . 2003 Aug 19 ; 128 1181 | DOI : https://doi.org/10.1039/B304902G

  • HR. Kermis
  • et al