Labeling-detection

HiLyte™ Fluor 555 succinimidyl ester (SE) - 1 mg

$249.00
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  • Cat.Number : AS-81251
  • Availability :
    In production
  • Shipping conditions : Ice fees will apply

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Quantity

HiLyte™ Fluor 555 acid, SE is an excellent amine-reactive fluorescent labeling dye that generates the protein conjugates that are only slightly red-shifted compared to those of Cy®3 dyes, resulting in an optimal match to filters designed for Cy®3 dyes. HiLyte™ Fluor 555 is more photostable than Cy®3, providing researchers with additional time for image capture. Additionally, our in-house data indicated that HiLyte™ Fluor 555 is superior to Cy®3 in fluorescence polarization-based assays. Extinction Coefficient (M-1cm-1): 150,000; Fluorescence quantum yield: 0.10; Fluorescence Life Time (ns): 0.3

Specifications

Chemistry
Molecular Mass/ Weight
  • 966.6
Properties
Absorbance (nm)
  • 552
Emission (nm)
  • 569
Color
Quantity & Purity
Purity
  • Peak Area by HPLC ≥90%
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • DMF or DMSO: Prepare 10 mg/ml dye stock solution in high quality anhydrous DMSO. Completely dissolve all dye content by vortexing. Dye solution should be prepared fresh, immediately before an experiment. Long-term storage in solution form may reduce dye activity. Any solution containing the dye should be protected from light.
Storage Conditions
  • -20°C Protected from Light
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Hyaluronan in cytosol—Microinjection-based probing of its existence and suggested functions.

Glycobiol . 2012 Oct 19 ; 23(2) 222 | DOI : 10.1093/glycob/cws149

  • H. Siiskonen