Protein

Human Sirtuin 1, recombinant

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  • Cat.Number : AS-72212
  • Availability :
    In production
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Histone deacetylases (HDACs) act as transcriptional repressors of genes catalyzing the removal of acetyl groups from a ε-N-acetyl lysine of histone. Sirtuin 1 (SIRT1), the human homolog of yeast Sir2 (Silent Information Regulator 2), is the most studied of the seven members of sirtuin family. SIRT1 has been implicated in several important cellular processes, including genomic stability and DNA repair, p53-mediated apoptosis, adipogenesis, and aging.

The recombinant human Sirtuin 1 (GenBank Accession #: NM_012238) with 193- 741 amino acids and GST tag at its N-terminal was expressed in E. coli. The molecular mass of the enzyme is approximately 87.2 kDa on SDS-PAGE. Its activity can be measured using enzymatic assay with a fluorogenic or FRET substrates

Specifications

Chemistry
UniProt number
  • Q96EB6
Molecular Mass/ Weight
  • 87.2 kDa
Modification
Conjugation
  • Unconjugated
Quantity & Purity
Concentration
  • lot dependent
Storage & stability
Form
  • In solution
Storage Buffer
  • 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 3 mM DTT.
Storage Conditions
  • Store at -80°C. Avoid repeated freeze-thaw cycles.
Activity
Application
Biomarker Target
Unit definition
  • One unit of enzyme deacetylates 1 picomole of Green Sirtuin 1 substrate, per minute at pH 8.0 at 37° C.
Specificity
  • human Sirtuin 1
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host
Source / Species
  • human

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