Labeling-detection

Protein A-HiLyte™ Fluor 488 Conjugate - 1 mg

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  • Cat.Number : AS-72235
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Protein A is a non-glycosylated cell wall protein of Staphylococcus aureus that can bind Fc part of immunoglobulin molecule of different species with strong affinity. Protein A consists of five IgG binding domains (A, B, C, D, and E), each is approximately 60 amino acids long with no cysteines present and two Staphylococcus aureus cell membrane binding domains (X and M).
AnaSpec's recombinant Protein A consists of only IgG binding domains and was expressed in E.coli. Its apparent molecular mass is approximately 30,000 Da compared to the most reported 42,000 Da for the native Protein A that is found in Staphylococcus aureus. Recombinant Protein A conjugated with HiLyte™ Fluor-488 can be used for immunoglobulin detection in IHC and IF and for assay development. Green Fluorescence. Excitation/Emission wavelength= 499 nm/523 nm

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References

Interaction between streptococcal IgG Fc receptors and human and rabbit IgG domains.

Immunol . 1986 Feb 01 ; 57 305 | DOI : 1453956

  • A.K. Schröder
  • et al

Structural Studies on the Four Repetitive Fc-Binding Regions in Protein A from Staphylococcus aureus

Eur J Biochem . 1977 Sep 01 ; 78(2) 471 | DOI : https://doi.org/10.1111/j.1432-1033.1977.tb11760.x

  • J. Sjödahl

Some Physicochemical Properties of Protein A from Staphylococcus aureus

Eur J Biochem . 1972 Sep 01 ; 29(3) 579 | DOI : https://doi.org/10.1111/j.1432-1033.1972.tb02024.x

  • I. Björk
  • et al