IMG-EN-AS-72237-01.jpg
Labeling-detection

Protein A-HiLyte™ Fluor 647 Conjugate - 1 mg

$184.00
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  • Cat.Number : AS-72237
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    In production
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Protein A is a non-glycosylated cell wall protein of Staphylococcus aureus that can bind Fc part of immunoglobulin molecule of different species with strong affinity. Protein A consists of five IgG binding domains (A, B, C, D, and E), each is approximately 60 amino acids long with no cysteines present and two Staphylococcus aureus cell membrane binding domains (X and M).
AnaSpec's recombinant Protein A consists of only IgG binding domains and was expressed in E.coli. Its apparent molecular mass is approximately 30,000 Da compared to the most reported 42,000 Da for the native Protein A that is found in Staphylococcus aureus. Recombinant Protein A conjugated with HiLyte™ Fluor-647 can be used for immunoglobulin detection in IHC and IF and for assay development. Near-infrared fluorescence. Excitation/Emission wavelength= 649 nm/ 674 nm

Specifications

Chemistry
Molecular Mass/ Weight
  • ~30000
Properties
Absorbance (nm)
  • 649
Emission (nm)
  • 674
Color
Quantity & Purity
Concentration
  • 1 mg/ml
Purity
  • >95% by SDS-PAGE
Storage & stability
Form
  • In solution
Storage Buffer
  • 10mM phosphate, 150mM sodium chloride, pH 7.2, 0.1% sodium azide as preservative
Storage Conditions
  • Stable for 2-3 months at 4°C. For long-term storage, divide the solution into aliquots and store at -20°C or add an equal volume of glycerol and store the solution at -20°C without aliquoting. Avoid multiple thaw-freeze cycles.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host
Source / Species
  • E. coli

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References

Interaction between streptococcal IgG Fc receptors and human and rabbit IgG domains.

Immunol . 1986 Feb 01 ; 57 305 | DOI : 1453956

  • A.K. Schröder
  • et al

Structural Studies on the Four Repetitive Fc-Binding Regions in Protein A from Staphylococcus aureus

Eur J Biochem . 1977 Sep 01 ; 78(2) 471 | DOI : https://doi.org/10.1111/j.1432-1033.1977.tb11760.x

  • J. Sjödahl

Some Physicochemical Properties of Protein A from Staphylococcus aureus

Eur J Biochem . 1972 Sep 01 ; 29(3) 579 | DOI : https://doi.org/10.1111/j.1432-1033.1972.tb02024.x

  • I. Björk
  • et al