Assay-kits

SensoLyte® 520 Cathepsin G Assay Kit Fluorimetric - 1 kit

$504.00
Check your price
  • Cat.Number : AS-72185
  • Availability :
    In stock
  • Shipping conditions : Ice fees will apply

Select specification

Quantity

Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin G is the serine protease released by neutrophils upon their activation. Cathepsin G is currently being explored as a target for anti-inflammatory and anti-infective drugs.
The SensoLyte® 520 Cathepsin G Activity Assay Kit uses a HiLyte™ Fluor 488/QXL™ 520 FRET peptide substrate for the measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte™ Fluor 488 is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490/520 nm. With superior fluorescence quantum yield and longer emission wavelength, the HiLyte™ Fluor 488/QXL™ 520 based FRET peptide has less interference from the autofluorescence of test compounds and cellular components and provides better assay sensitivity. The fluorescent signal from HiLyte Fluor™ 488 is stable at low pH, which is the optimal pH for cathepsin activity. The kit can be used to detect the activity of Cathepsin G enzyme in biological samples and purified enzyme preparations.

Specifications

Packaging
Kits components
  • Component A: QXL™ 520/HiLyte™ Fluor 488, Cathepsin G substrate, Ex/Em=490 nm/520 nm upon cleavage: 55 µL Component B: HiLyte™ Fluor 488, fluorescence reference standard, Ex/Em=490 nm/520 nm: 200 µM, 10 µL Component C: Cathepsin G, Human Neutrophil: 0.1 mg/mL, 15 µL Component D: Assay Buffer: 25 mL Component E: Cathepsin G Inhibitor: 1 mM, 20 µL
Chemistry
UniProt number
  • P08311
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all kit components at -20°C, except for Component C. Store Component C at -80°C. Protect Components A and B from light and moisture. Component D can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

You may also be interested in the following product(s)

IMG-EN-AS-60740-01.jpg

hBD-1, b-Defensin-1, human - 0.1 mg

Cat.Number : AS-60740
$318.00
IMG-EN-AS-61302-01.jpg

LL-37, Antimicrobial Peptide, human - 1 mg

Cat.Number : AS-61302
$188.00
IMG-EN-AS-62554-01.jpg

Myosin H Chain Fragment (614-631), mouse - 1 mg

Cat.Number : AS-62554
$110.00

Citations

Serine protease inhibitor A3 in atherosclerosis and aneurysm disease.

Int J Mol Med. . 2012 May 09 ; 30(2) 288 | DOI : 10.3892/ijmm.2012.994

  • D. Wågsäter
  • et al