SensoLyte® 520 TMPRSS2 Activity Assay Kit *Fluorimetric* - 1 kit

TMPRSS2 is part of the type 2 transmembrane serine protease (TTSP) family, which includes 19 trypsin-like serine proteases. These enzymes are primarily found on cell surfaces and play critical roles in various biological processes. TMPRSS2 (transmembrane protease, serine 2) is indeed a crucial factor in the entry of certain viruses, including SARS-CoV, MERS-CoV, and various influenza viruses like H7N9 and H1N1. Targeting TMPRSS2 could provide a promising antiviral strategy, as inhibiting its activity may block the viral entry process. Research is ongoing to develop specific inhibitors that can effectively disrupt TMPRSS2 function without adversely affecting normal cellular processes. This approach could pave the way for new treatments for both coronavirus and specific influenza infections, enhancing our ability to manage these viral diseases. TMPRSS2 is also known to be highly expressed in metastatic prostate cancer and is regulated by androgen receptor signaling, making it a potentially valuable target to study tumor growth, metastasis and treatment of the disease.

The TMPRSS2 Assay Kit is an assay for screening of enzyme inhibitors or for continuous assay of TMPRSS2 activity using a fluorogenic substrate. Upon cleavage by TMPRSS2, this substrate generates the Rh110 (Rhodamine 110) fluorophore which has a bright green fluorescence and can be detected at excitation/emission=496 nm/520 nm. The longer wavelength spectra and higher extinction coefficient of Rh110 provide greater sensitivity and less interference from other reaction components.

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