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Assay-kits

SensoLyte® ADHP Hydrogen Peroxide Assay Kit Fluorimetric - 1 kit

$255.00
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  • Cat.Number : AS-71112
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Hydrogen peroxide is involved in a number of biological events; in particular; free radical-induced biochemical reactions. SensoLyte® ADHP Hydrogen Peroxide Assay Kit uses highly purified ADHP to quantify hydrogen peroxide in solutions; in cell extracts and in live cells. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions. The kit contains: ADHP phosphatase substrate (Ex/Em=573/590 nm upon oxidation); Calibration standard; Reaction buffer; Stop buffer; An optimized ‘mix and read’ assay protocol that is compatible with HTS liquid handling instruments

Specifications

Packaging
Kits components
  • Component A: ADHP: 10 mM, 250 µL Component B: H2O2 standard: 1 vial Component C: Assay buffer: 60 mL Component D: HRP, Horseradish peroxidase: 5 vials, 100 µL/vial
Properties
Absorbance (nm)
  • 573
Emission (nm)
  • 590
Storage & stability
Storage Conditions
  • Store all components at -20°C. Component C can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 1 uM
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Identification of a small-molecule inhibitor of the interaction between Survivin and Smac/DIABLO

Biochem Biophys Res Commun . 2010 Feb 01 ; 393(2) 253 | DOI : 10.1016/j.bbrc.2010.01.113

  • T. Oikawa

Ricin A-chain requires c-Jun N-terminal kinase to induce apoptosis in nontransformed epithelial cells

Int J Biochem Cell Biol . 2009 Aug 18 ; 41(12) 2503 | DOI : 10.1016/j.biocel.2009.08.007

  • A. Jetzt

Evidence of a common mechanism of disassembly of adherens junctions through Gα13 targeting of VE-cadherin

J Exp Med. . 2014 Mar 03 ; 211(3) 579 | DOI : 10.1084/jem.20131190

  • H. Gong