IMG-EN-AS-72159-01.jpg
Assay-kits

SensoLyte® AFC Urokinase (uPA) Activity Assay Kit Fluorimetric - 1 kit

$565.00
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  • Cat.Number : AS-72159
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Urokinase-type plasminogen activator (uPA) is a serine protease that functions in the conversion of the zymogen plasminogen to the active, broad-spectrum serine protease plasmin. Plasmin, in turn, mediates the pericellular proteolysis of extracellular matrix components and activates other proteases such as matrix metalloproteinases and collagenases that lead to the further degradation and remodeling of the extracellular matrix. Besides the proteolytic function, upon binding to uPA receptor (uPAR), uPA is involved in initiating versatile intracellular signal pathways that regulate cell proliferation, adhesion, and migration through its interaction with various integrins and vitronectin. uPA has been implicated in a number of normal and pathological processes, and recognized as a prognostic marker for various cancers as well as a target for drug discovery.
The SensoLyte AFC Urokinase (uPA) Assay Kit is optimized for screening of enzyme inhibitors. This kit contains a fluorogenic substrate with a high reactivity and low background. uPA cleaves the substrate resulting in release of AFC (7-amino-4-trifluoromethylcoumarin) fluorophore. Fluorescence can be monitored at excitation /emission= 380/500 nm.

Specifications

Packaging
Kits components
  • Component A: uPA Substrate, Ex/Em=380/500 nm upon enzyme cleavage: 2 mM, 50 µL Component B: AFC, Fluorescence Reference Standard, Ex/Em=380/500 nm: 2 mM, 10 µL Component C: 2X Assay Buffer: 10 mL Component D: Purified Human uPA: 5 Units/µL, 40 µL Component E: uPA Inhibitor: 1 mM, 100 µL
Chemistry
UniProt number
  • P00749
Properties
Absorbance (nm)
  • 380
Emission (nm)
  • 500
Storage & stability
Storage Conditions
  • Store component D at -80°C. Store all other components at -20°C. Component C can be stored at room temperature for convenience. Protect components A and B from light and moisture.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Tazarotene-Induced Gene 1 (TIG1) Interacts with Serine Protease Inhibitor Kazal-Type 2 (SPINK2) to Inhibit Cellular Invasion of Testicular Carcinoma Cells

Biomed Res Int. . 2019 Jan 01 ; 2019 6171065 | DOI : https://doi.org/10.1155/2019/6171065

  • RY. Shyu
  • et al

In Vivo Functional Evaluation of Tissue-Engineered Vascular Grafts Fabricated Using Human Adipose-Derived Stem Cells from High Cardiovascular Risk Populations

Tissue Eng Part A. . 2016 May 01 ; 22(9-10) 765 | DOI : 10.1089/ten.tea.2015.0379

  • JT. Krawiec
  • et al