SensoLyte® Green Protease Assay Kit Fluorimetric - 1 kit
- Cat.Number : AS-71124
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The SensoLyte® Green Protease Assay Kit is widely used for detection of generic protease activities. The kit uses a casein derivative that is heavily labeled with a rhodamine derivative, resulting in almost total quenching of the conjugate's fluorescence. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly green fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity (Ex/Em=488/520 nm). The SensoLyte® protease assay kits do not require any separation steps and can be used to continuously measure the kinetics of a variety of exopeptidases and endopeptidases.
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Citations
Antioxidant and trypsin inhibition activity of dioscorin influenced by yam cultivars and eluent pH
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- W-T. Kao
A plant pathogen virulence factor inhibits the eukaryotic proteasome by a novel mechanism
Nature . 2008 Apr 10 ; 452(7188) 755 | DOI : 10.1038/nature06782
- M. Groll
Inhibitory effects of antrodins A–E from Antrodia cinnamomea and their metabolites on hepatitis C virus protease
Phytother Res . 2009 Apr 01 ; 23(4) 582 | DOI : 10.1002/ptr.2657
- D.T. Phuong
Synthesis of dammarane-type triterpene derivatives and their ability to inhibit HIV and HCV proteases
Bioorg Med Chem . 2009 Mar 14 ; 17(8) 3003 | DOI : 10.1016/j.bmc.2009.03.019
- Y. Wei