SensoLyte® Green Protease Assay Kit Fluorimetric - 1 kit

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  • Cat.Number : AS-71124
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The SensoLyte® Green Protease Assay Kit is widely used for detection of generic protease activities. The kit uses a casein derivative that is heavily labeled with a rhodamine derivative, resulting in almost total quenching of the conjugate's fluorescence. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly green fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity (Ex/Em=488/520 nm). The SensoLyte® protease assay kits do not require any separation steps and can be used to continuously measure the kinetics of a variety of exopeptidases and endopeptidases.


Kits components
  • Component A: Protease substrate: HiLyte Fluor™ 488-labeled casein, Ex/Em=488 nm/520 nm upon cleavage: 280 µL Component B: Trypsin 5 U/µL, 100 µL Component C: 2X Assay buffer: 30 mL
Absorbance (nm)
  • 488
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store component A and B at -20°C. Protect component A from light and moisture. Component C can be stored at room temperature for convenience.
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
  • Research use

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