Description Quantity Component A Assay Buffer 25 ml Component B Beta-Amyloid (1-40) (Aβ40), human 0.5 mg (2 x 0.25 mg, net peptide) Component C Thioflavin T (ThT...
The assay is based on the property of ThT dye to increase its fluorescence Ex/Em=440/484 nm when bound to aggregated Aβ peptides. Figure 1....
Component A should be kept at 4 °C to prevent premature Aβ40 aggregation. 1.1 ThT working solution: Add 100 µl of 20mM ThT (Component C) to 900 µl of the

Description Quantity Component A Assay Buffer 25 ml Component B Beta-Amyloid (1-42) (Aβ42), human 0.5 mg (2 x 0.25 mg, net peptide) Component C Thioflavin T (ThT...
The assay is based on the property of ThT dye to increase its fluorescence Ex/Em=440/484 nm when bound to aggregated Aβ peptides. Figure 1....
Component A should be kept at 4 °C to prevent premature Aβ40 aggregation. 1.1 ThT working solution: Add 100 µl of 20mM ThT (Component C) to 900 µl of the

Transgenic mice brain lysates were also or plain buffer was mixed with ThT at 10 μM final concentration....
3.0 3) Aβ42 samples pre-incubated with inhibitor or Na bicarbonate buffer were diluted to a final concentra- results with conventional Thioflavin T (ThT...
ThT Fibrillation Assay Summary Aβ42 Aggregation ELISA Summary 6 Expected Measured Assay Principle 5 Inhibitor % Aggregated Aβ42, pg/ml Aβ42, pg/ml 4 No

Description: AggreSure™ beta-Amyloid (1-42) peptide was pretreated and tested for ® aggregation using SensoLyte ThT Aβ42 Aggregation kit (Cat.

Description: AggreSure™ beta-Amyloid (1-40) peptide was pretreated and tested for ® aggregation using SensoLyte ThT Aβ40 Aggregation kit (Cat.