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Fluorescent Dyes  >  Fluorescent Physiological and Neurological Indicators  >>  Calcein; AM *UltraPure Grade* *Small Package*

Product Name Calcein; AM *UltraPure Grade* *Small Package*
CAS 148504-34-1
Size 50 µg x 20
Catalog # AS-89204
US$ $271.95
Purity ≥95% by HPLC

Calcein, AM is a cell-permeant and non-fluorescent compound that is widely used for determining cell viability. In live cells the non-fluorescent calcein, AM is hydrolyzed by intracellular esterases into the strongly green fluorescent anion calcein. The fluorescent calcein is well-retained in the cytoplasm in live cells. Hela cells stained with AnaSpec’s Calcein AM fluorescent dye (Cat# AS-89201, AS-89202, AS-89203 and AS-89204).

MCF-7 cells grown in 8-well slide chamber for 2 days. Cells were washed twice before adding 1 uM Calcein AM (AnaSpec cat# AS-89202). Images acquired every 3 seconds and total elapsed time on video is 12 min.

Images Calcein; AM *UltraPure Grade* *Small Package*
Detailed Information Material Safety Data Sheets (MSDS)
Storage -20°C desiccated and protected from light
Molecular Weight 994.857
Molecular Formula C46H46N2O23
Spectral Properties Abs/Em = 494/517 nm
Solvent System DMSO
Product Citations Decaris, ML. et al. (2010). Design of experiments approach to engineer cell-secreted matrices for directing osteogenic differentiation. Annals Biomed Eng 39, 1174.

Hernlem, B. et al. (2010). Dual fluorochrome flow cytometric assessment of yeast viability. Current Microbiol 61, 57.

Zaveri, T. et al. (2010). Contributions of surface topography and cytotoxicity to the macrophage response to zinc oxide nanorods. Biomater 31, 2999.

Zhang, K-H. et al. (2009). Ferritin heavy chain–mediated iron homeostasis and subsequent increased reactive oxygen species production are essential for epithelial-mesenchymal transition. Cancer Res. 10.1158/0008-5472.CAN-09-0112.

Decaris, ML. et al. (2009). Influence of the oxygen microenvironment on the proangiogenic potential of human endothelial colony forming cells. Angiogenesis 12, 303.

Delaunay, JL. et al. (2009). A missense mutation in ABCB4 gene involved in progressive familial intrahepatic cholestasis type 3 leads to a folding defect that can be rescued by low temperature. Hepatology 49, 1218.
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