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Labeling-detection

AnaTag™ HiLyte™ Fluor 555 Microscale Protein Labeling Kit - 1 kit

$300.00
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  • Cat.Number : AS-72046-
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HiLyte™ Fluor 555 acid, SE is an excellent amine-reactive fluorescent labeling dye that generates the protein conjugates that are only slightly red-shifted compared to those of Cy® 3 dyes, resulting in an optimal match to filters designed for Cy® dyes. The HiLyte™ Fluor 555-protein conjugates are very stable and suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications. Additionally, our in-house data indicated that HiLyte™ Fluor 555 is superior to Cy® 3 in fluorescence polarization-based assays. The kit has all essential components for performing the conjugation reaction and for purifying the HiLyte™ Fluor 555-protein conjugate. The entire process takes only half an hour. The kit is able to perform three conjugation reactions with each reaction labeling up to 200 µg IgG. Kit Size: 3 labeling reactions (3 x 200 µg protein).
Note: AnaTag kits are not intended for peptide labeling.

Specifications

Packaging
Kits components
  • Component A: HiLyte™ Fluor 555 SE Amino-reactive dye: 3 vials Component B: Reaction buffer for pH adjustment of the conjugation reaction: 0.5 mL Component C: Spin column to purify dye-protein conjugate: 3 pre-packed columns Component D: DMSO solvent for preparing dye stock solution: 150 µL Component E: Elution buffer for eluting dye-protein conjugate: 20 mL Component F: Wash tube to hold buffer for Spin column: 3 tubes Component G: Collect tube to collect dye-protein conjugate: 3 tubes
Properties
Absorbance (nm)
  • 553
Emission (nm)
  • 568
Storage & stability
Storage Conditions
  • Store component A at -20°C. Store all other components at 4°C. Protect component A from light and moisture.
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Piezoelectric printing and probing of Lectin NanoProbeArrays for glycosylation analysis.

Biochem Biophys Res Comm. . 2008 Aug 19 ; 375(4) 526 | DOI : 10.1016/j.bbrc.2008.08.033

  • VJ. Nagaraj