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Assay Kits  >  Viral/Bacterial Protease Assay Kits - SARS-CoV2/COVID-19, HIV, HCV, TEV, WNV, Sortase, Furin  >>  SensoLyte ® 440 West Nile Virus Protease Assay Kit *Fluorimetric*

Product Name SensoLyte ® 440 West Nile Virus Protease Assay Kit *Fluorimetric*
Size 1 kit
Catalog # AS-72079
US$ $511

West Nile virus (WNV) causes severe neurological disease and fatalities in both human and animal hosts. There is currently no effective vaccine or antiviral drug to protect against WNV infection. The West Nile virus NS3 protease, essential for the proteolytic processing of the viral polyprotein precursor, is a promising target for the development of anti-WNV drugs. The SensoLyte® 440 West Nile Virus Protease Assay Kit provides a convenient homogenous assay for high throughput screening of WNV protease inhibitors and for continuous assay of NS3 protease activity using a fluorogenic Pyr-RTKR-AMC peptide. Upon NS3 protease cleavage, Pyr-RTKR-AMC generates the AMC fluorophore that has bright blue fluorescence and can be detected at excitation/emission=354 nm/442 nm. The kit contains: • AMC labeled WNV protease substrate (Ex/Em=354/442 nm upon cleavage) • Assay buffer • WNV protease inhibitor • Fluorescence reference standard for calibration • A detailed protocol

500 assays (96-well plate)

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Product Citations Samanta, S. et al. (2012). Discovery, synthesis, and in vitro evaluation of West Nile Virus protease inhibitors based on the 9,10-Dihydro-3H,4aH-1,3,9,10a-tetraazaphenanthren-4-one scaffold. Chem Med Chem 7, 1210. doi: 10.1002/cmdc.201200136

Assenberg, R. et al. (2009). Crystal Structure of a Novel Conformational State of the Flavivirus NS3 Protein: Implications for Polyprotein Processing and Viral Replication. J Virol 83, 12895.
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